血浆外泌体作为上皮性卵巢癌的诊断生物标志物。

Plasma exosomal and as diagnostic biomarkers in epithelial ovarian cancer.

机构信息

Post-Doctoral Research Station, Tianjin Medical University, Tianjin.

Department of Gynecological Oncology, Shandong Cancer Hospital & Institute, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, Shandong, 250117, PR China.

出版信息

Future Oncol. 2022 Aug;18(26):2919-2931. doi: 10.2217/fon-2022-0321. Epub 2022 Jul 27.

DOI:10.2217/fon-2022-0321

PMID:35893704

Abstract

The study aimed to clarify the diagnostic value of exosomal miRNAs in epithelial ovarian cancer (EOC). Plasma exosomes were isolated from peripheral blood of EOC patients and healthy donors by ultracentrifugation and verified by transmission electron microscopy, qNano and western blot. The expression of exosomal miRNAs was detected by quantitative PCR, and the diagnostic efficiency of exosomal miRNAs was evaluated by receiver operating characteristic analysis. Exosomal , and were significantly decreased in EOC as compared with healthy controls. The area under the curve of the combination of three exosomal miRNAs was 0.8337. Moreover, the level of exosomal was related to the neutrophil-lymphocyte ratio, which decreased in EOC patients with a high neutrophil-lymphocyte ratio. Exosomal , and act as potentially EOC diagnostic and prognostic biomarkers.

摘要

本研究旨在阐明外泌体 miRNA 在卵巢上皮性癌（EOC）中的诊断价值。通过超速离心从 EOC 患者和健康供体的外周血中分离血浆外泌体，并通过透射电子显微镜、qNano 和 Western blot 进行验证。通过定量 PCR 检测外泌体 miRNA 的表达，并通过接受者操作特征分析评估外泌体 miRNA 的诊断效率。与健康对照组相比，EOC 中外泌体、和明显降低。三种外泌体组合的曲线下面积为 0.8337。此外，外泌体的水平与中性粒细胞-淋巴细胞比值有关，在中性粒细胞-淋巴细胞比值高的 EOC 患者中降低。外泌体、和可作为潜在的 EOC 诊断和预后生物标志物。