Institute of Food and Nutrition Development, Ministry of Agriculture and Rural Affairs, Beijing 100081, China.
Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Nutrients. 2022 Jul 22;14(15):3014. doi: 10.3390/nu14153014.
Profound malnutrition and immunodeficiency are serious negative effects of radiotherapy and bone marrow transplantation for hematologic malignancy patients. This study aimed to evaluate the effects of nutritional supplementation with a soy-whey protein mixture on hematopoietic and immune reconstitution in an allogeneic transplant mouse model. Male BALB/c (H-2Kd) mice, 6-8 weeks-old, were divided randomly into five groups and then provided with different protein nutrition support. After 28 days, blood samples, bone marrow, spleen, and thymus were harvested to measure the effects. The results showed that soy-whey blended protein supplements promoted hematopoietic stem cell engraftment, body weight recovery, and the recovery of white blood cells, lymphocytes, and neutrophils; triggered the expansion of hematopoietic stem cells and progenitor cell pools by increasing the numbers of the c-kit progenitor, Lin-Sca1c-kit, short-term hematopoietic stem cells, and multipotent progenitors; enhanced thymus re-establishment and splenic subset recovery in both organ index and absolute number; improved overall nutritional status by increasing total serum protein, albumin, and globulin; protected the liver from radiation-induced injury, and increased antioxidant capacity as indicated by lower concentrations of alanine aminotransferase, aspartate aminotransferase, malondialdehyde, and 4-hydroxynonenal. This study indicated that soy-whey blended protein as important nutrients, from both plant and animal sources, had a greater positive effect on patients with hematological malignancies to accelerate hematopoiesis and immune reconstitution after bone marrow transplantation.
严重的营养不良和免疫缺陷是血液恶性肿瘤患者放疗和骨髓移植的严重副作用。本研究旨在评估大豆-乳清蛋白混合物的营养补充对同种异体移植小鼠模型中造血和免疫重建的影响。雄性 BALB/c（H-2Kd）小鼠，6-8 周龄，随机分为五组，然后给予不同的蛋白质营养支持。28 天后，采集血液样本、骨髓、脾脏和胸腺，以测量其效果。结果表明，大豆-乳清混合蛋白补充剂促进了造血干细胞的植入、体重恢复以及白细胞、淋巴细胞和中性粒细胞的恢复；通过增加 c-kit 祖细胞、Lin-Sca1c-kit、短期造血干细胞和多能祖细胞的数量，触发了造血干细胞和祖细胞池的扩增；增强了胸腺重建和脾亚群恢复，器官指数和绝对数量均增加；通过增加总血清蛋白、白蛋白和球蛋白来改善整体营养状况；通过降低丙氨酸氨基转移酶、天冬氨酸氨基转移酶、丙二醛和 4-羟壬烯醛的浓度，保护肝脏免受辐射损伤，提高抗氧化能力。本研究表明，大豆-乳清混合蛋白作为重要的营养物质，来自植物和动物来源，对血液恶性肿瘤患者具有更大的积极作用，可加速骨髓移植后造血和免疫重建。
