可见光不会引起人真皮成纤维细胞的 DNA 损伤。

Visible red light does not induce DNA damage in human dermal fibroblasts.

机构信息

Department of Dermatology, State University of New York, Downstate Health Sciences University, Brooklyn, New York, USA.

Dermatology Service, VA New York Harbor Healthcare System - Brooklyn Campus, Veterans Hospital, Brooklyn, New York, USA.

出版信息

J Biophotonics. 2022 Nov;15(11):e202200023. doi: 10.1002/jbio.202200023. Epub 2022 Aug 25.

DOI:10.1002/jbio.202200023

PMID:35894162

Abstract

Visible red light (RL) therapy is a rapidly expanding treatment option for dermatological conditions, including acne, psoriasis and chronic wounds. It is currently unknown if high fluences of RL induce DNA damage via reactive oxygen species (ROS) stress or other pathways. Our lab previously demonstrated that RL generates ROS in human dermal fibroblasts (HDFs). Other studies show that UV and blue light generate ROS and DNA damage in fibroblasts. This study aims to determine if RL induces DNA damage in HDFs. We found that 320 J/cm , 640 J/cm and 1280 J/cm RL (633 ± 6 nm) did not measurably increase DNA damage in the form of cyclobutane pyrimidine dimers (CPD) or 6-4 photoproducts (6-4PP) immediately, 3 hours and 24 hours following irradiation. Our study further supports that RL therapy is safe in human skin fibroblasts.

摘要

可见红光（RL）疗法是一种治疗皮肤病的新兴治疗方法，包括痤疮、银屑病和慢性伤口。目前尚不清楚高剂量 RL 是否通过活性氧（ROS）应激或其他途径引起 DNA 损伤。我们实验室之前的研究表明，RL 在人真皮成纤维细胞（HDFs）中产生 ROS。其他研究表明，UV 和蓝光在成纤维细胞中产生 ROS 和 DNA 损伤。本研究旨在确定 RL 是否会在 HDFs 中引起 DNA 损伤。我们发现，320 J/cm、640 J/cm 和 1280 J/cm RL（633 ± 6nm）在照射后立即、3 小时和 24 小时，以环丁烷嘧啶二聚体（CPD）或 6-4 光产物（6-4PP）的形式，未检测到可测量的 DNA 损伤。我们的研究进一步支持 RL 疗法在人类皮肤成纤维细胞中是安全的。