Department of Dermatology, SUNY Downstate Health Sciences University, Brooklyn, New York, USA.
Dermatology Service, New York Harbor Veterans Affairs Healthcare System-Brooklyn Campus, Brooklyn, New York, USA.
J Biophotonics. 2024 Feb;17(2):e202300388. doi: 10.1002/jbio.202300388. Epub 2023 Dec 12.
Photobiomodulation (PBM) can be used to treat a range of conditions in dermatology. PBM refers to the changes induced by red (RL, 620-700 nm) and near-infrared (NIR, 700-1440 nm) light. Light radiation-induced DNA damage is a major contributor to aging and skin cancer. It is crucial to study the effects of PBM on DNA to ensure safety. Our lab previously demonstrated that RL (633 ± 6 nm) did not result in human dermal fibroblasts (HDFs) DNA damage. This study employed similar methods to investigate NIR effects. Commercially available LED-NIR (830 ± 5 nm) panels (66, 132, and 264 J/cm ) did not result in DNA damage measured by cyclobutane pyrimidine dimers and pyrimidine-6,4-pyrimidone photoproducts in HDFs compared to temperature-matched controls immediately, 3 h, and 24 h following irradiation and compared to positive and negative controls. This demonstrates that LED-NIR does not damage DNA in HDFs in vitro.
光生物调节（PBM）可用于治疗皮肤科的多种疾病。PBM 是指由红光（RL，620-700nm）和近红外光（NIR，700-1440nm）引起的变化。光辐射诱导的 DNA 损伤是导致衰老和皮肤癌的主要原因。因此，研究 PBM 对 DNA 的影响以确保安全性至关重要。我们实验室之前的研究表明，RL（633±6nm）不会导致人真皮成纤维细胞（HDFs）的 DNA 损伤。本研究采用类似的方法来研究 NIR 的影响。与温度匹配的对照相比，商用 LED-NIR（830±5nm）面板（66、132 和 264J/cm2）在照射后立即、3 小时和 24 小时，以及与阳性和阴性对照相比，均未导致 HDFs 中的 DNA 损伤，通过环丁烷嘧啶二聚体和嘧啶-6,4-嘧啶酮光产物进行测量。这表明 LED-NIR 不会在体外损伤 HDFs 的 DNA。
