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新技术大规模研究神经肽。

New Technologies to Investigate Neuropeptides at Scale.

机构信息

Institute of Pharmacology and Toxicology and Neuroscience Center Zurich, University of Zürich, Zürich CH-8057, Switzerland.

Neuroscience Center Zurich, University and ETH Zürich, Zürich CH-8057, Switzerland.

出版信息

ACS Chem Neurosci. 2022 Aug 17;13(16):2353-2355. doi: 10.1021/acschemneuro.2c00394. Epub 2022 Jul 27.

DOI:10.1021/acschemneuro.2c00394
PMID:35894205
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7613611/
Abstract

Neuropeptides are some of the most elusive molecules to monitor in neuroscience. Detecting their release and spread in brain tissue requires the development and use of advanced technologies that enable specific neuropeptide measurements with high spatial and temporal resolution. This Viewpoint highlights some of the emerging tools and techniques that are already advancing our knowledge of neuropeptide physiology and discusses possible future developments.

摘要

神经肽是神经科学中最难监测的分子之一。要检测其在脑组织中的释放和扩散,需要开发和使用先进的技术,这些技术能够以高时空分辨率进行特定的神经肽测量。本观点强调了一些新兴的工具和技术,这些工具和技术正在推动我们对神经肽生理学的认识,并讨论了可能的未来发展。

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New Technologies to Investigate Neuropeptides at Scale.新技术大规模研究神经肽。
ACS Chem Neurosci. 2022 Aug 17;13(16):2353-2355. doi: 10.1021/acschemneuro.2c00394. Epub 2022 Jul 27.
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Detection of neuropeptides in vivo and open questions for current and upcoming fluorescent sensors for neuropeptides.体内神经肽的检测以及当前和未来神经肽荧光传感器的未解决问题。
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Current and emerging methods for probing neuropeptide transmission.当前和新兴的神经肽传递探测方法。
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Coordinated RNA-Seq and peptidomics identify neuropeptides and G-protein coupled receptors (GPCRs) in the large pine weevil Hylobius abietis, a major forestry pest.协调的 RNA-Seq 和肽组学鉴定大型松象鼻虫 Hylobius abietis 中的神经肽和 G 蛋白偶联受体 (GPCRs),这是一种主要的林业害虫。
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引用本文的文献

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NeuroScale: evolutional scale-based protein language models enable prediction of neuropeptides.NeuroScale:基于进化尺度的蛋白质语言模型可实现神经肽预测。
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本文引用的文献

1
Probing Neuropeptide Volume Transmission In Vivo by Simultaneous Near-Infrared Light-Triggered Release and Optical Sensing.通过近红外光触发释放和光学感应同时探测体内神经肽容量传递。
Angew Chem Int Ed Engl. 2022 Aug 22;61(34):e202206122. doi: 10.1002/anie.202206122. Epub 2022 Jul 8.
2
A genetically encoded sensor for in vivo imaging of orexin neuropeptides.一种用于活体成像的内源性大麻素神经肽的基因编码传感器。
Nat Methods. 2022 Feb;19(2):231-241. doi: 10.1038/s41592-021-01390-2. Epub 2022 Feb 10.
3
Characterization of a Multiple-Scan-Rate Voltammetric Waveform for Real-Time Detection of Met-Enkephalin.用于实时检测 Met-Enkephalin 的多扫描速率伏安波形的特性。
ACS Chem Neurosci. 2019 Apr 17;10(4):2022-2032. doi: 10.1021/acschemneuro.8b00351. Epub 2019 Jan 12.
4
Pro-opiomelanocortin (POMC) as homeostatic control system.阿黑皮素原(POMC)作为稳态控制系统。
Ann N Y Acad Sci. 1987;512:328-37. doi: 10.1111/j.1749-6632.1987.tb24971.x.