Division of Cardiovascular Medicine, Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden.
Center for Molecular Medicine, Karolinska Institutet, Stockholm, Sweden.
Adipocyte. 2022 Dec;11(1):413-419. doi: 10.1080/21623945.2022.2104514.
With obesity and its comorbidities continuing to rise, we urgently need to improve our understanding of what mechanisms trigger the white adipose tissue to become dysfunctional in response to over-feeding. The recent invent of 3D culturing models has produced several noteworthy protocols for differentiating unilocular adipocytes , promising to revolutionize the obesity research field by providing more representative adipose tissue models for such mechanistic studies. In parallel, these 3D models provide important insights to how profoundly the microenvironment influences adipocyte differentiation and morphology. This commentary highlights some of the most recent 3D models, including (HUVASs), developed by our lab. We discuss recent developments in the field, provide further insights to the importance of the microvasculature for adipocyte maturation, and summarize what challenges remain to be solved before we can achieve a culture model that fully recapitulates all aspects of human white adipocyte biology . Taken together, the commentary highlights important recent advances regarding 3D adipocyte culturing and underlines the many advantages these models provide over traditional 2D cultures, with the aim of convincing more laboratories to switch to 3D models.
随着肥胖及其合并症的持续上升，我们迫切需要更好地理解是什么机制导致白色脂肪组织在过度喂养时发生功能障碍。最近，3D 培养模型的发明产生了几种用于分化单房脂肪细胞的引人注目的方案，有望通过为这种机制研究提供更具代表性的脂肪组织模型来彻底改变肥胖研究领域。与此同时，这些 3D 模型还深入了解了微环境对脂肪细胞分化和形态的影响。本文述评重点介绍了我们实验室最近开发的一些最新的 3D 模型，包括（HUVASs）。我们讨论了该领域的最新进展，进一步探讨了微血管系统对于脂肪细胞成熟的重要性，并总结了在我们能够实现完全再现人类白色脂肪细胞生物学所有方面的培养模型之前仍然存在的挑战。总之，述评强调了 3D 脂肪细胞培养的重要最新进展，并强调了这些模型相对于传统 2D 培养的许多优势，目的是说服更多的实验室转向 3D 模型。
