不同组别儿童隐孢子虫病诊断及寄生虫种特征。
Cryptosporidium diagnosis in different groups of children and characterization of parasite species.
机构信息
Universidade Federal da Bahia, Programa de Pós-Graduação Stricto Sensu em Farmácia, Salvador, BA, Brasil.
Universidade Federal da Bahia, Hospital Professor Edgard Santos, Salvador, BA, Brasil.
出版信息
Rev Soc Bras Med Trop. 2022 Jul 25;55:e00412022. doi: 10.1590/0037-8682-0041-2022. eCollection 2022.
Abstract
BACKGROUND
Microscopy and enzyme-linked immunosorbent assay (ELISA) are routinely used for Cryptosporidium diagnosis, without differentiating the parasite species.
METHODS
Children's feces were analyzed by modified Ziehl-Neelsen (mZN) and ELISA for Cryptosporidium diagnosis and by polymerase chain reaction-restriction fragment length polymorphism for species identification.
RESULTS
Cryptosporidium frequency was 2.6%. The sensitivity and specificity of ELISA were 85.7% and 99.7%, respectively, with excellent concordance with mZN (kappa=0.854). Parasite species were characterized as Cryptosporidium hominis (78.3%), Cryptosporidium felis (17.4%), and Cryptosporidium parvum (4.3%).
CONCLUSIONS
Coproantigen ELISA is as efficient as mZN for Cryptosporidium diagnosis. Cryptosporidium genotyping suggests anthroponotic and zoonotic transmission to children.
摘要
背景
显微镜检查和酶联免疫吸附试验（ELISA）常用于隐孢子虫病的诊断，但无法区分寄生虫的种类。
方法
改良齐尔-尼尔森染色法（mZN）和 ELISA 检测儿童粪便中的隐孢子虫，聚合酶链反应-限制性片段长度多态性检测用于鉴定寄生虫的种类。
结果
隐孢子虫的检出率为 2.6%。ELISA 的灵敏度和特异性分别为 85.7%和 99.7%，与 mZN 的一致性极好（kappa=0.854）。寄生虫的种类分别为：人隐孢子虫（78.3%）、猫隐孢子虫（17.4%）和微小隐孢子虫（4.3%）。
结论
隐孢子虫 coproantigen ELISA 与 mZN 一样，可有效用于隐孢子虫病的诊断。隐孢子虫基因分型提示存在人传人及动物传人途径。
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