Department of Otorhinolaryngology, Qilu Hospital of Shandong University, Jinan, China.
NHC Key Laboratory of Otorhinolaryngology, Shandong University, Jinan, Shandong 250012, China.
Curr Protein Pept Sci. 2022;23(6):424-436. doi: 10.2174/1389203723666220727090317.
Hypopharyngeal squamous cell carcinoma (HSCC) is a common malignant cancer characterized by high metastasis and infiltration. The development of new approaches for the early diagnosis and identification of new therapeutic targets is essential. TIPE2 is well known as a tumor suppressor and related to a favorable prognosis of HSCC. However, its underlying mechanism remains unclear.
TIPE2 expression was determined by immunohistochemistry and RT-qPCR. A TIPE2 overexpression stable cell line was generated by lentivirus infection. TIPE2 and other related protein levels were detected by western blotting. The cell cycle and apoptosis were performed by flow cytometric analysis. Cell proliferation was measured with a Cell Counting Kit-8 (CCK-8) assay, and the activity of caspase-3 and caspase-7 was assessed by Caspase-Glo® 3/7 Assay. All data were analyzed with SPSS 25 and GraphPad Prism 8.0.
TIPE2 expression was significantly down-regulated in HSCC. Low TIPE2 expression may be associated with poor prognosis in HSCC. TIPE2 overexpression markedly inhibited tumor cell migration. Moreover, TIPE2 decreased cell proliferation but promoted apoptosis. TIPE2 suppressed tumor growth by activating Epithelial-Mesenchymal Transition (EMT) and the extrinsic apoptosis pathway.
TIPE2 inhibited tumor progression by suppressing cell migration but promoting apoptosis. TIPE2 can be a new therapeutic target in HSCC.
下咽鳞状细胞癌（HSCC）是一种常见的恶性肿瘤，其特征为高转移和浸润。开发新的方法进行早期诊断和鉴定新的治疗靶点至关重要。TIPE2 是一种众所周知的肿瘤抑制因子，与 HSCC 的良好预后相关。然而，其潜在机制尚不清楚。
通过免疫组织化学和 RT-qPCR 测定 TIPE2 的表达。通过慢病毒感染生成 TIPE2 过表达稳定细胞系。通过 Western blot 检测 TIPE2 和其他相关蛋白水平。通过流式细胞术分析检测细胞周期和凋亡。通过细胞计数试剂盒-8（CCK-8）测定法测量细胞增殖，通过 Caspase-Glo® 3/7 测定法评估 caspase-3 和 caspase-7 的活性。所有数据均使用 SPSS 25 和 GraphPad Prism 8.0 进行分析。
TIPE2 在 HSCC 中表达显著下调。低 TIPE2 表达可能与 HSCC 的不良预后相关。TIPE2 过表达显著抑制肿瘤细胞迁移。此外，TIPE2 降低细胞增殖但促进细胞凋亡。TIPE2 通过激活上皮间质转化（EMT）和外在凋亡途径抑制肿瘤生长。
TIPE2 通过抑制细胞迁移但促进细胞凋亡抑制肿瘤进展。TIPE2 可以成为 HSCC 的新治疗靶点。
