School of Pharmacy, Jiangxi University of Chinese Medicine, Nanchang, Jiangxi 330004, P. R. China.
Chinese Pharmacopoeia Commission, Beijing 100061, P. R. China.
Mol Omics. 2022 Sep 26;18(8):765-778. doi: 10.1039/d2mo00158f.
Lotus leaves have the dual identity of medicine and food homology as included in the Chinese Pharmacopoeia. Nuciferine is the major bioactive component which is highly abundant in the leaves of . Nuciferine has been shown to potentially improve energy metabolism and protect neurons in cerebral ischemia. However, the mechanisms underlying the protective effects of nuciferine on acute ischemic stroke (AIS) are still unclear. Metabolomics was used for uncovering the underlying therapeutic mechanism of nuciferine in AIS with the help of H NMR. The rat model of AIS was generated by the occlusion of the middle cerebral artery (MCAO). After treatment with nuciferine, several indexes of oxidative stress and inflammation, such as total antioxidant capacity (T-AOC), total glutathione/oxidized glutathione (GSH/GSSG), malondialdehyde (MDA), superoxide dismutase (SOD), tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were significantly improved, and some metabolic biomarkers (histidine, glycine, glycerol, serine, tyrosine, lysine, choline, ) were significantly regulated. Bioinformatic analysis demonstrated that these biomarkers and the derived genes (myeloperoxidase, catalase, ), fatty acid and amino acid metabolisms and 9 key metabolic pathways were involved in the nuciferine activity, which indicated the potential therapeutic mechanisms of nuciferine in AIS.
荷叶作为《中国药典》收载的药食同源品种，具有双重身份。荷叶中的主要生物活性成分之一是荷叶碱，其在荷叶中的含量非常丰富。荷叶碱具有改善能量代谢和保护脑缺血神经元的作用。然而，荷叶碱对急性缺血性脑卒中（AIS）的保护作用机制尚不清楚。本研究采用^1H-NMR 代谢组学技术，探讨荷叶碱治疗 AIS 的作用机制。采用大脑中动脉阻塞（MCAO）法制备 AIS 大鼠模型，用荷叶碱进行干预。结果表明，荷叶碱可显著改善氧化应激和炎症相关指标，如总抗氧化能力（T-AOC）、总谷胱甘肽/氧化型谷胱甘肽（GSH/GSSG）、丙二醛（MDA）、超氧化物歧化酶（SOD）、肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β），并显著调节一些代谢生物标志物（组氨酸、甘氨酸、甘油、丝氨酸、酪氨酸、赖氨酸、胆碱等）。生物信息学分析表明，这些代谢物及其相关基因（髓过氧化物酶、过氧化氢酶等）、脂肪酸和氨基酸代谢以及 9 条关键代谢通路参与了荷叶碱的活性，提示了荷叶碱治疗 AIS 的潜在作用机制。
