Department of Biochemistry, Melaka Manipal Medical College (Manipal Campus), Manipal Academy of Higher Education, Manipal, India.
Department of Anatomy, Manipal-TATA Medical College, Jamshedpur, Manipal Academy of Higher Education, Manipal, India.
J Food Biochem. 2021 Apr;45(4):e13690. doi: 10.1111/jfbc.13690. Epub 2021 Mar 22.
This study evaluates the modulation of inflammatory markers by petroleum ether fraction of Trigonella foenum-graecum L. (PE-TFG) seed extract in ovariectomized rats. The HPTLC method was used for standardization and to quantify the diosgenin in PE-TFG. For testing PE-TFG in rats, the total duration of treatment was 12-weeks, and the rats were sacrificed on week 12. The tissue samples such as blood, liver, heart, and aorta were isolated for testing inflammatory markers such as adiponectin, leptin, PPAR-γ, TNF-α, lipid profile, hepatic markers, antioxidants, and oxidative stress markers. The PE-TFG treatment decreased the elevation of total cholesterol, triglyceride, AST, and ALT. Upon PE-TFG treatment, there was a significant increase in adiponectin and PPAR-γ mRNA expression. Leptin and TNF-α were normal after treatment with PE-TFG seed extract. Further, micro-steatosis of hepatocytes marked glomerular hypertrophy in the kidney and increased thickness of tunica intima and media of common carotid artery was reversed after treatment with PE-TFG. PRACTICAL APPLICATIONS: Trigonella foenum-graecum L. is a curative plant used to treat inflammatory conditions like diabetes, obesity, dyslipidemia, arthritis, cancer, and digestive disorders. In our study, PE-TFG supplementation has a protective effect on OVX-induced inflammation, oxidative stress, mRNA expression of adiponectin and PPAR-γ, hepatic steatosis, and decreased thickness of tunica intima and media of common carotid artery.
这项研究评估了鹰嘴豆石油醚提取物（PE-TFG）对去卵巢大鼠炎症标志物的调节作用。HPTLC 方法用于标准化并定量测定 PE-TFG 中的薯蓣皂苷元。为了在大鼠中测试 PE-TFG，总治疗时间为 12 周，第 12 周处死大鼠。分离血、肝、心和主动脉等组织样本，以测试炎症标志物，如脂联素、瘦素、PPAR-γ、TNF-α、血脂谱、肝标志物、抗氧化剂和氧化应激标志物。PE-TFG 治疗降低了总胆固醇、甘油三酯、AST 和 ALT 的升高。经 PE-TFG 治疗后，脂联素和 PPAR-γ mRNA 表达显著增加。PE-TFG 种子提取物治疗后瘦素和 TNF-α正常。此外，PE-TFG 治疗后，肾小球肥大的肝细胞微脂肪变性和颈总动脉内膜和中膜厚度增加得到逆转。
鹰嘴豆是一种治疗炎症的药用植物，可用于治疗糖尿病、肥胖症、血脂异常、关节炎、癌症和消化疾病等炎症。在我们的研究中，PE-TFG 补充剂对 OVX 诱导的炎症、氧化应激、脂联素和 PPAR-γ mRNA 表达、肝脂肪变性以及颈总动脉内膜和中膜厚度降低具有保护作用。
