Centre for Interdisciplinary Research in Basic Sciences, International Islamic University, Islamabad, Pakistan.
PLoS One. 2022 Jul 27;17(7):e0271445. doi: 10.1371/journal.pone.0271445. eCollection 2022.
A 42-days experiment was conducted on a day old birds (n = 400) to evaluate the effect of enzyme supplements in feed on the growth, blood parameters, phosphorous content in bones, and nitrogen retention. Different treatments included: control (C) without enzyme supplement, while the other three groups included enzyme mixture T1 and T2 with two commercially available enzyme mix, and T3 with indigenously produced multi-enzyme complex from Bacillus subtilis KT004404. Birds that were fed with indigenously produced multi-enzyme complex showed significant weight gain as compared to other groups. The total feed intake of the birds fed with enzyme supplements was higher than the birds in the control group. The feed conversion ratio was significantly improved (p < 0.05) in treatment groups (T1, T2, T3) as compared to the control. The blood parameters which were analyzed included uric acid, triglycerides, total cholesterol, and serum proteins i.e. globulin and albumin. Birds fed with the enzyme in the group T1, T2 and T3 exhibited higher (p < 0.05) body weight gain. Tibia ash content was significantly higher (p < 0.05) in T1, T2, and T3 as compared to the control. The results of the current study indicate that supplementing poultry feed with the exogenous multi-enzyme produced from Bacillus subtilis KT004404 improved the growth of the birds, feed utilization, and exhibited beneficial effects on the blood parameters, phosphorous and nitrogen retention in broiler chicken.
一项为期 42 天的实验在 1 日龄雏鸡（n = 400）身上进行，以评估饲料中添加酶制剂对生长、血液参数、骨骼磷含量和氮保留的影响。不同的处理包括：对照组（C）不添加酶制剂，而其他三组包括两种市售酶混合物 T1 和 T2，以及 T3 是从枯草芽孢杆菌 KT004404 中产生的本土多酶复合物。与其他组相比，饲喂本土多酶复合物的鸡只体重显著增加。饲喂酶制剂的鸡只总采食量高于对照组。与对照组相比，处理组（T1、T2、T3）的饲料转化率显著提高（p < 0.05）。分析的血液参数包括尿酸、甘油三酯、总胆固醇和血清蛋白，即球蛋白和白蛋白。T1、T2 和 T3 组饲喂酶的鸡只体重增加更高（p < 0.05）。与对照组相比，T1、T2 和 T3 组胫骨灰分含量显著更高（p < 0.05）。本研究结果表明，在禽饲料中添加枯草芽孢杆菌 KT004404 产生的外源多酶可改善鸡只的生长、饲料利用，并对肉鸡的血液参数、磷和氮保留产生有益影响。
