Thelin S, Hultman J, Ronquist G, Hansson H E
Scand J Thorac Cardiovasc Surg. 1987;21(1):39-45. doi: 10.3109/14017438709116917.
Creatine phosphate is a precursor molecule for ATP synthesis, even under ischemic conditions. We investigated its functional and metabolic effects when added to cardioplegic solution. Rat hearts were subjected to normothermic ischemia for 15 or 30 min and then freeze-clamped. During ischemia there was gradual reduction of high-energy phosphates, but the hearts with creatine phosphate supplement showed higher myocardial content of ATP and of the creatine compound. Other hearts, subjected to 20 min of ischemia, were reperfused with blood for 40 min. Creatine phosphate supplementation resulted in better left ventricular isovolumic work during spontaneous activity, but in paced activity (400 beats/min) no significant differences were seen. After reperfusion, supplemented hearts showed a tendency to higher levels of ATP and creatine phosphate. In all three groups the hearts with cardioplegic supplement had significantly increased myocardial content of pyruvate without proportional lactate increase. The results indicate that creatine phosphate may be an effective constituent in cardioplegic solution.
即使在缺血条件下，磷酸肌酸也是三磷酸腺苷（ATP）合成的前体分子。我们研究了将其添加到心脏停搏液中时的功能和代谢作用。对大鼠心脏进行15或30分钟的常温缺血，然后进行冷冻钳夹。在缺血期间，高能磷酸盐逐渐减少，但补充了磷酸肌酸的心脏显示出较高的心肌ATP含量和肌酸化合物含量。其他心脏进行20分钟缺血后，再灌注血液40分钟。补充磷酸肌酸导致自发活动期间左心室等容功更好，但在起搏活动（400次/分钟）中未观察到显著差异。再灌注后，补充组的心脏显示出ATP和磷酸肌酸水平有升高的趋势。在所有三组中，添加心脏停搏液的心脏心肌丙酮酸含量显著增加，而乳酸没有成比例增加。结果表明，磷酸肌酸可能是心脏停搏液中的一种有效成分。
