Fujian Key Laboratory of Genetics and Breeding of Marine Organisms, College of Ocean and Earth Sciences, Xiamen University, Xiamen, China.
State Key Laboratory of Large Yellow Croaker Breeding, Ningde Fufa Fisheries Company Limited, Ningde, China.
Mar Biotechnol (NY). 2022 Aug;24(4):681-689. doi: 10.1007/s10126-022-10135-x. Epub 2022 Jul 27.
The large yellow croaker (Larimichthys crocea) plays an economically vital role in the marine aquaculture in China. Suffering from infection of bacteria and protozoon, effect of extreme weather and stress from high-density farming, genome editing is thought to be an important tool applied to L. croea for enhancing commercial traits such as growth rate, disease resistance, and nutrition component. In this study, we identified two mstn genes in L. croea and investigated the different phylogenetic clades, gene structures, and conserved syntenic relationships. To obtain fast-growing large yellow croaker, we specially selected two validated targets for mstnb knockout, which was homologous to mammalian myostatin gene (MSTN) and downregulated skeletal muscle growth and development. Five significant mutation types were generated in two mosaic mutants by transferring specific CRISPR/Cas9 RNPs (ribonucleoprotein) into the one-cell fertilized embryos based on CRISPR/Cas9 technology. Subsequently, we also elucidated the obstacles and possible measures to improve the success rate of inducing modified large yellow croaker. Our results would provide valuable method and reference for facilitating genome editing programs of the large yellow croaker in the future.
大黄鱼（Larimichthys crocea）在中国的海水养殖业中扮演着至关重要的经济角色。由于受到细菌和原生动物的感染、极端天气的影响以及高密度养殖带来的压力，基因编辑被认为是一种重要的工具，可用于大黄鱼，以提高生长速度、抗病能力和营养成分等商业特性。在这项研究中，我们在大黄鱼中鉴定了两个 MSTN 基因，并研究了不同的系统发育分支、基因结构和保守的同线性关系。为了获得生长迅速的大黄鱼，我们特别选择了两个已验证的 MSTN b 敲除靶点，这些靶点与哺乳动物肌肉生长抑制素基因（MSTN）同源，可下调骨骼肌的生长和发育。通过基于 CRISPR/Cas9 技术将特定的 CRISPR/Cas9 RNPs（核糖核蛋白）转移到单细胞受精卵中，在两个嵌合体突变体中产生了五种显著的突变类型。随后，我们还阐明了提高改良大黄鱼诱导成功率的障碍和可能措施。我们的研究结果将为未来大黄鱼的基因组编辑计划提供有价值的方法和参考。
