Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-Environment, Fisheries College, Jimei University, Xiamen 361021, China.
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China.
Int J Mol Sci. 2022 Jul 27;23(15):8298. doi: 10.3390/ijms23158298.
The large yellow croaker () is one of the most economically valuable mariculture fish in China. Infection of can cause an outbreak of "internal organ white-spot disease", which seriously affects the aquaculture of the large yellow croaker. Ubiquitylation is closely related to the post-translation modification of proteins and plays a vital role in many hosts' immune defense pathways, while the E2-binding enzyme is a key factor in ubiquitination. Our previous genome-wide association study found that the ubiquitin-binding enzyme E2G1 (designed ) was one of the candidate genes related to disease resistance in large yellow croaker. In this study, we analyzed the molecular characteristics, function, and immune mechanism of the . The full-length cDNA is 812 bp, with an open reading frame of 513 bp, encoding 170 amino acid residues. The results of the RT-qPCR and immunohistochemistry analysis revealed that its transcription and translation were significantly activated by the infection of in large yellow croaker. Immunocytochemistry experiments verified the co-localization of UBE2G1 and the ubiquitin proteins in the head kidney cells of large yellow croaker. Through GST pull-down, we found that UBE2G1 interacted with NEDD8 to co-regulate the ubiquitination process. The above results indicate that UBE2G1 is essential in the regulation of ubiquitination against infection in large yellow croaker, which lays a foundation for further study on the resistance mechanism of internal organ white-spot disease.
大黄鱼（）是中国最具经济价值的海水养殖鱼类之一。感染（）可引起“内脏白点病”的爆发，严重影响大黄鱼的养殖业。泛素化与蛋白质的翻译后修饰密切相关，在许多宿主的免疫防御途径中发挥着重要作用，而 E2 结合酶是泛素化的关键因素。我们之前的全基因组关联研究发现，泛素结合酶 E2G1（设计为）是与大黄鱼抗病性相关的候选基因之一。在这项研究中，我们分析了 的分子特征、功能和免疫机制。其全长 cDNA 为 812bp，开放阅读框为 513bp，编码 170 个氨基酸残基。RT-qPCR 和免疫组织化学分析的结果表明，在大黄鱼感染后，其转录和翻译明显被激活。免疫细胞化学实验验证了 UBE2G1 和泛素蛋白在大黄鱼头肾细胞中的共定位。通过 GST 下拉实验，我们发现 UBE2G1 与 NEDD8 相互作用，共同调节泛素化过程。上述结果表明，UBE2G1 在大黄鱼抵御感染的泛素化调节中至关重要，为进一步研究内脏白点病的抗病机制奠定了基础。
