Institute of Hybrid Wheat, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
Molecular Genetic Beijing Key Laboratory of Hybrid Wheat, Beijing 100097, China.
Int J Mol Sci. 2022 Jul 22;23(15):8057. doi: 10.3390/ijms23158057.
Temperature-sensitive genic male sterile (TGMS) line Beijing Sterility 366 (BS366) has been utilized in hybrid breeding for a long time, but the molecular mechanism underlying male sterility remains unclear. Expression arrays, small RNA, and degradome sequencing were used in this study to explore the potential role of miRNA in the cold-induced male sterility of BS366. Microspore observation showed defective cell plates in dyads and tetrads and shrunken microspores at the vacuolated stage. Differential regulation of Golgi vesicle transport, phragmoplast formation, sporopollenin biosynthesis, pollen exine formation, and lipid metabolism were observed between cold and control conditions. Pollen development was significantly represented in the 352 antagonistic miRNA-target pairs in the integrated analysis of miRNA and mRNA profiles. The specific cleavage of and by miR160 and miR393 were found in the cold-treated BS366 degradome, respectively. Thus, the cold-mediated miRNAs impaired cell plate formation through repression of Golgi vesicle transport and phragmoplast formation. The repressed expression of and impaired pollen exine formation. The results of this study will contribute to our understanding of the roles of miRNAs in male sterility in wheat.
温度敏感型基因雄性不育（TGMS）系北京不育 366（BS366）长期以来一直被用于杂种优势育种，但雄性不育的分子机制尚不清楚。本研究利用表达谱芯片、小 RNA 和降解组测序技术，探讨了 miRNA 在 BS366 低温诱导雄性不育中的潜在作用。小孢子观察显示二分体和四分体中的细胞板缺陷，液泡化阶段的小孢子收缩。在冷处理和对照条件下，观察到高尔基体囊泡运输、成膜体形成、花粉外壁生物合成、花粉外壁形成和脂类代谢的差异调节。miRNA 和 mRNA 谱综合分析显示，在 352 对拮抗 miRNA-靶对中，花粉发育得到了很好的表达。在冷处理的 BS366 降解组中发现了 miR160 和 miR393 对 和 的特异性切割。因此，低温介导的 miRNA 通过抑制高尔基体囊泡运输和成膜体形成来破坏细胞板的形成。 和 的表达受抑制，破坏了花粉外壁的形成。本研究结果将有助于我们理解 miRNA 在小麦雄性不育中的作用。
