CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
Laboratory for Marine Drugs and Bioproducts, Pilot National Laboratory for Marine Science and Technology (Qingdao), No. 1 Wenhai Road, Qingdao 266237, China.
Mar Drugs. 2021 Jun 25;19(7):366. doi: 10.3390/md19070366.
As a popular marine saccharide, chitooligosaccharides (COS) has been proven to have good antioxidant activity. Its antioxidant effect is closely related to its degree of polymerization, degree of acetylation and sequence. However, the specific structure-activity relationship remains unclear. In this study, three chitosan dimers with different sequences were obtained by the separation and enzymatic method, and the antioxidant activity of all four chitosan dimers were studied. The effect of COS sequence on its antioxidant activity was revealed for the first time. The amino group at the reducing end plays a vital role in scavenging superoxide radicals and in the reducing power of the chitosan dimer. At the same time, we found that the fully deacetylated chitosan dimer showed the strongest DPPH scavenging activity. When the amino groups of the chitosan dimer were acetylated, it showed better activity in scavenging hydroxyl radicals. Research on COS sequences opens up a new path for the study of COS, and is more conducive to the investigation of its mechanism.
作为一种受欢迎的海洋糖，壳寡糖（COS）已被证明具有良好的抗氧化活性。其抗氧化作用与其聚合度、乙酰化度和序列密切相关。然而，具体的结构-活性关系尚不清楚。在这项研究中，通过分离和酶法获得了三种具有不同序列的壳聚糖二聚体，并研究了所有四种壳聚糖二聚体的抗氧化活性。首次揭示了 COS 序列对其抗氧化活性的影响。还原端的氨基在清除超氧自由基和壳聚糖二聚体的还原能力方面起着至关重要的作用。同时，我们发现完全脱乙酰化的壳聚糖二聚体对 DPPH 自由基的清除活性最强。当壳聚糖二聚体的氨基乙酰化时，其清除羟基自由基的活性更好。对 COS 序列的研究为 COS 的研究开辟了新途径，更有利于对其机制的研究。
