State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2, West Yuan Ming Yuan Road, Beijing 100193, China.
Int J Mol Sci. 2022 Jul 26;23(15):8239. doi: 10.3390/ijms23158239.
The white-backed planthopper (WBPH), , is one of the most important piercing-sucking pests of rice () in Asia. Mucin-like salivary protein (SFMLP) is highly expressed in the salivary glands of WBPH, which plays an important role in WBPH feeding. In this study, WBPH injected with had difficulty in sucking phloem sap from rice plants, which significantly reduced their food intake, weight, and survival. In contrast, the knockdown of the gene had only a marginal effect on the survival of WBPH fed an artificial diet. Further studies showed that silencing resulted in the short and single-branched salivary sheaths secretion and less formation of salivary flanges in rice. These data suggest that is involved in the formation of the salivary sheath and is essential for feeding in WBPH. Overexpression of the gene in rice plants promoted the feeding of WBPH, whereas silencing the gene in rice plants significantly decreased WBPH performance. Additionally, it was found that overexpression of in rice plants elicited the signalling pathway of SA (salicylic acid) while suppressing JA (jasmonic acid); in contrast, silencing of the gene in rice plants showed the opposite results. This study clarified the function of in WBPH feeding as well as mediating rice defences.
白背飞虱（WBPH）是亚洲水稻上最重要的刺吸式害虫之一。黏蛋白样唾液蛋白（SFMLP）在 WBPH 的唾液腺中高度表达，在 WBPH 取食中发挥重要作用。在本研究中，注射 的 WBPH 难以从水稻植株中吸取韧皮部汁液，这显著降低了它们的食物摄入量、体重和存活率。相比之下， 基因的敲低对以人工饲料为食的 WBPH 的存活率只有微小的影响。进一步的研究表明，沉默 基因导致短而单支的唾液鞘分泌，并减少水稻中唾液凸缘的形成。这些数据表明 参与唾液鞘的形成，是 WBPH 取食所必需的。在水稻植株中过表达 基因促进了 WBPH 的取食，而在水稻植株中沉默该基因则显著降低了 WBPH 的性能。此外，还发现水稻植株中 基因的过表达诱导了 SA（水杨酸）信号通路，同时抑制了 JA（茉莉酸）；相反，沉默水稻中 基因则表现出相反的结果。本研究阐明了 基因在 WBPH 取食以及介导水稻防御中的功能。
