Wang Huiyu, Zheng Yushan, Wang Meiyun, Liu Wusheng, Li Ying, Xiao Dong, Liu Tongkun, Hou Xilin
State Key Laboratory of Crop Genetics & Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (East China), Ministry of Agriculture and Rural Affairs of China, Engineering Research Center of Germplasm Enhancement and Utilization of Horticultural Crops, Ministry of Education of China, Nanjing Agricultural University, No.1 Weigang Road, Xuanwu District, Nanjing 210095, China.
Department of Plant Science and Technology, Beijing Key Laboratory of New Technology in Agricultural Application, National Demonstration Center for Experimental Plant Production Education, Beijing University of Agriculture, No.7 Beinong Road, Changping District, Beijing 102206, China.
Hortic Res. 2024 Sep 28;12(1):uhae280. doi: 10.1093/hr/uhae280. eCollection 2025 Jan.
Root development is a complex process involving phytohormones and transcription factors. Our previous research has demonstrated that is significantly expressed in Bok choy roots under salt stress, and heterologous expression of increases salt tolerance and promotes root development in transgenic . However, the precise molecular mechanisms by which BcWRKY33A governs root development remain elusive. Here, we investigated the role of in both root elongation and root hair formation in transgenic Bok choy roots. Our data indicated that overexpression of stimulated root growth and stabilized root hair morphology, while silencing prevented primary root elongation and resulted in abnormal root hairs morphology. Meanwhile, our research uncovered that BcWRKY33A directly binds to the promoters of and , leading to an upregulation of their expression. In transgenic Bok choy roots, increased and transcript levels improved primary root elongation and root hair formation, respectively. Additionally, we pinpointed as a NaCl-responsive gene that directly stimulates the expression of in response to salt stress. All results shed light on the regulatory mechanisms governing root development by BcWRKY25-BcWRKY33A-BcLRP1/BcCOW1 module and propose potential strategies for improving salt tolerance in Bok choy.
根系发育是一个涉及植物激素和转录因子的复杂过程。我们之前的研究表明，[此处原文缺失具体基因名称]在盐胁迫下的小白菜根系中显著表达，并且[此处原文缺失具体基因名称]的异源表达提高了转基因植物的耐盐性并促进了根系发育。然而，BcWRKY33A调控根系发育的精确分子机制仍不清楚。在这里，我们研究了[此处原文缺失具体基因名称]在转基因小白菜根系的根伸长和根毛形成中的作用。我们的数据表明，[此处原文缺失具体基因名称]的过表达刺激了根的生长并稳定了根毛形态，而[此处原文缺失具体基因名称]的沉默则阻止了主根伸长并导致根毛形态异常。同时，我们的研究发现BcWRKY33A直接结合[此处原文缺失具体基因名称]和[此处原文缺失具体基因名称]的启动子，导致它们的表达上调。在转基因小白菜根系中，[此处原文缺失具体基因名称]和[此处原文缺失具体基因名称]转录水平的增加分别改善了主根伸长和根毛形成。此外，我们确定[此处原文缺失具体基因名称]是一个NaCl响应基因，它在盐胁迫下直接刺激[此处原文缺失具体基因名称]的表达。所有结果揭示了BcWRKY25 - BcWRKY33A - BcLRP1/BcCOW1模块调控根系发育的机制，并提出了提高小白菜耐盐性的潜在策略。
