Key Laboratory for Pesticide Residue Detection of Ministry of Agriculture and Rural Affairs, Institute of Quality and Standard of Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Department of Chemistry, Zhejiang Sci-Tech University, Hangzhou 310018, China.
Food Chem. 2021 May 1;343:128490. doi: 10.1016/j.foodchem.2020.128490. Epub 2020 Oct 28.
Dendrobium officinale Kimura et Migo (D. officinale) is a dual-use plant with both botanical medicine and food applications, drawing increasing attentions. Pesticides are inevitably applied on D. officinale in commercial artificial-sheltered cultivation, yet little is known about pesticide residue levels in D. officinale. A modified high through-put QuEChERS method coupled with HPLC-MS/MS was developed and validated to detect 76 pesticides in D. officinale. Graphitized multi-wall carbon nanotubes (g-MWCNTs) was selected as the clean-up sorbent, showing relative weak affinity to triazole fungicide having planar structure in their molecular and low matrix effects of pesticides in D. officinale samples compared to MWCNTs and pesticarb. The validated method was applied to analyze pesticide residues in 86 real D. officinale samples collected from three main producing provinces. 43 different pesticides were detected with highest residue of 6.11 mg/kg for dimethomorph. Given possible health risks related to pesticide residues, accordingly, risk assessment of human exposure to pesticides via intake of D. officinale was thus performed, indicating that the pesticide residue in fresh or dry D. officinale would not cause potential risk to human health either in the long-term or short-term. This work improved our understanding of potential exposure risk of pesticide multi-residues in D. officinale.
铁皮石斛（Dendrobium officinale Kimura et Migo）是一种兼具植物药和食品应用的两用植物，受到越来越多的关注。在商业人工遮荫栽培中，不可避免地会在铁皮石斛上使用农药，但对于铁皮石斛中的农药残留水平知之甚少。本研究建立了一种改良的高通量 QuEChERS 方法，并结合 HPLC-MS/MS 对铁皮石斛中的 76 种农药残留进行了检测。与 MWCNTs 和农残吸附剂相比，石墨化多壁碳纳米管（g-MWCNTs）作为净化吸附剂对具有平面结构的三唑类杀菌剂具有相对较弱的亲和力，对铁皮石斛样品中的农药基质效应较低。该方法已应用于分析来自三个主要产区的 86 个实际铁皮石斛样品中的农药残留。共检测到 43 种不同的农药，其中联苯菌胺的残留量最高，为 6.11mg/kg。鉴于与农药残留有关的健康风险，因此对通过摄入铁皮石斛而接触农药对人体的暴露风险进行了评估，结果表明新鲜或干燥的铁皮石斛中的农药残留不会对人体健康造成潜在风险，无论是长期还是短期。这项工作提高了我们对铁皮石斛中多农药残留潜在暴露风险的认识。
