Hattori Hiroyuki, Moriyama Akihiro, Ohno Tomoki, Shibata Takahiro, Iwahashi Hitoshi, Mitsunaga Tohru
Asian Satellite Campuses Institute, Nagoya University, Nagoya 464-8601, Japan.
Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan.
Food Chem (Oxf). 2022 Jul 18;5:100123. doi: 10.1016/j.fochms.2022.100123. eCollection 2022 Dec 30.
Obesity is now recognized as an epidemic, requiring rapid intervention. We previously demonstrated that vanilloids from the African spice known as Grains of Paradise (GOP) exhibit strong anti-obesity effects. Here, the molecular mechanism behind the obesity prevention property of the GOP extract was investigated by employing molecular networking-based lipid profiling and 16S rRNA sequencing. Administration of either GOP extract or two of its components, 6-paradol and 6-gingerol, reversed the unbalanced gut microbiota composition induced by a high-fat diet (HFD) with a decrease in Firmicutes / Bacteroidetes ratio and increase in genera and . Moreover, GOP extract ameliorated abnormal fatty acid metabolism in gut bacteria. Furthermore, lipid molecular networking revealed differences in lipid composition, such as triglycerides and bile acids, in the feces. These results suggest that 6-paradol and 6-gingerol can restore an unhealthy gut environment and prevent lipid absorption, a possible consequence of the reconstruction of the gut microbiota.
肥胖如今被视为一种流行病，需要迅速干预。我们之前证明，来自非洲香料天堂谷（GOP）的香草酸酯具有很强的抗肥胖作用。在此，通过基于分子网络的脂质谱分析和16S rRNA测序，研究了GOP提取物预防肥胖特性背后的分子机制。给予GOP提取物或其两种成分——6-对甲氧基苯基-2,3-环氧-1,6-二氧杂螺[4.4]壬烷（6-paradol）和6-姜酚，可逆转高脂饮食（HFD）诱导的肠道微生物群组成失衡，厚壁菌门/拟杆菌门比例降低，[此处原文缺失两个属名]属增加。此外，GOP提取物改善了肠道细菌中异常的脂肪酸代谢。此外，脂质分子网络揭示了粪便中脂质组成的差异，如甘油三酯和胆汁酸。这些结果表明，6-对甲氧基苯基-2,3-环氧-1,6-二氧杂螺[4.4]壬烷（6-paradol）和6-姜酚可以恢复不健康的肠道环境并防止脂质吸收，这可能是肠道微生物群重建的一个结果。
