College of Food Science and Nutritional Engineering, China Agricultural University, Haidian District, No.17, Qinghua East Road, Beijing, 100083, China.
National Engineering Research Center for Fruit and Vegetable Processing, Beijing, 100083, China.
Eur J Nutr. 2020 Dec;59(8):3617-3634. doi: 10.1007/s00394-020-02196-2. Epub 2020 Feb 11.
Obesity, a strong risk factor for metabolic disorder, has become a major impediment for public health globally. The objective of this study was to assess the anti-obesity effect of mung bean, and the relationship between the gut microbiota modulatory effects of mung bean and the prevention of obesity.
Thirty-two four-week-old male C57BL/6 J mice were divided into four groups: normal chow diet (NCD), high-fat diet (HFD), a high-fat diet supplemented with 30% whole mung bean flour (HFD-WMB), and a high-fat diet supplemented with 30% decorticated mung bean flour (HFD-DMB). The ability of a mung bean-based diet to combat obesity-related metabolic disorder was determined by assessing the changes in physiological, histological, biochemical parameters, and gut microbiota composition of mice with HFD-induced obesity at 12 weeks.
Both of WMB and DMB supplementation can effectively alleviate HFD-induced lipid metabolic disorders, which was accompanied by a reduction in hepatic steatosis. However, the only supplementation with WMB significantly reduced HFD-induced body weight gain, fat accumulation, and adipocyte size, and ameliorated the glucose tolerance and insulin resistance by sensitizing insulin action. Furthermore, high-throughput pyrosequencing of 16S rRNA revealed that WMB and DMB supplementation could normalize HFD-induced gut microbiota dysbiosis. Especially, WMB and DMB supplementation significantly promoted the relative abundance of Akkermansia and Bifidobacterium, respectively, and both of them significantly restored the relative abundance of several HFD-dependent taxa back to normal status in this study. Spearman's correlation analysis revealed that those genera are closely correlated with obesity-related indices.
Although WMB showed better beneficial effects on HFD-induced obesity in comparison with DMB, DMB still retained some health benefits. Moreover, the alleviation of HFD-induced changes by mung bean supplementation was, at least, partially conciliated by structural modulation of gut microbiota.
肥胖是代谢紊乱的一个重要危险因素，已成为全球公共卫生的主要障碍。本研究旨在评估绿豆的抗肥胖作用，以及绿豆对肠道微生物群的调节作用与肥胖预防之间的关系。
将 32 只 4 周龄雄性 C57BL/6J 小鼠分为 4 组：正常饲料组（NCD）、高脂肪饮食组（HFD）、高脂肪饮食添加 30%全绿豆粉组（HFD-WMB）和高脂肪饮食添加 30%去皮绿豆粉组（HFD-DMB）。通过评估 12 周时 HFD 诱导肥胖的小鼠生理、组织学、生化参数和肠道微生物群组成的变化，来确定基于绿豆的饮食对抗肥胖相关代谢紊乱的能力。
WMB 和 DMB 的补充均可有效缓解 HFD 诱导的脂质代谢紊乱，伴随肝脂肪变性减少。然而，只有 WMB 的补充可显著减轻 HFD 诱导的体重增加、脂肪堆积和脂肪细胞增大，并通过增强胰岛素作用来改善葡萄糖耐量和胰岛素抵抗。此外，16S rRNA 高通量焦磷酸测序显示，WMB 和 DMB 的补充可使 HFD 诱导的肠道微生物群失调正常化。特别是，WMB 和 DMB 的补充分别显著促进 Akkermansia 和双歧杆菌的相对丰度，并且它们都显著将依赖于 HFD 的几个分类群的相对丰度恢复到本研究中的正常状态。Spearman 相关性分析显示，这些菌属与肥胖相关指标密切相关。
尽管 WMB 与 DMB 相比对 HFD 诱导的肥胖具有更好的有益效果，但 DMB 仍保留一些健康益处。此外，绿豆补充缓解 HFD 诱导的变化至少部分通过肠道微生物群的结构调节来协调。
