Ni Jiahui, Zhu Peng, Mo Qilang, Luo Wei, Du Zongjun, Jiang Jun, Yang Song, Zhao Liulan, Gong Quan, Wang Yan
College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
Fisheries Institute, Sichuan Academy of Agricultural Sciences, Chengdu, China.
Front Physiol. 2022 Jul 11;13:928858. doi: 10.3389/fphys.2022.928858. eCollection 2022.
Liver is an important organ for glucose and lipid metabolism, immunity, and detoxification in fish. However, the gene regulatory network of postnatal liver development still remains unknown in teleost fish. In this study, we performed transcriptome analysis on the liver of at three stages. A total of 1692 differentially expressed genes (DGEs) were identified across three liver developmental stages. The oil red O staining and PAS staining revealed that the lipid content of liver was increased and the glycogen content of liver was decreased during liver development. The fatty acids biosynthesis related genes were upregulated in adult and young stages compared with juvenile stage, while lipid degradation related genes were downregulated. The genes related to glycolysis, gluconeogenesis and glycogenolysis were upregulated in juvenile or young stages compared with adult stage. Further pathway analysis indicated that the CYP450 pathway, cell cycle and amino acid metabolic pathway were induced in the process of liver maturation. Our study presents the gene expression pattern in different liver development stages of and may guide future studies on metabolism of liver.
肝脏是鱼类进行葡萄糖和脂质代谢、免疫及解毒的重要器官。然而，硬骨鱼产后肝脏发育的基因调控网络仍不清楚。在本研究中，我们对三个阶段的 肝脏进行了转录组分析。在三个肝脏发育阶段共鉴定出1692个差异表达基因（DGE）。油红O染色和PAS染色显示，在肝脏发育过程中肝脏脂质含量增加而糖原含量降低。与幼鱼阶段相比，脂肪酸生物合成相关基因在成鱼和幼鱼阶段上调，而脂质降解相关基因下调。与糖酵解、糖异生和糖原分解相关的基因在幼鱼或幼鱼阶段与成鱼阶段相比上调。进一步的通路分析表明，CYP450通路、细胞周期和氨基酸代谢通路在肝脏成熟过程中被诱导。我们的研究展示了 不同肝脏发育阶段的基因表达模式，并可能为未来 肝脏代谢的研究提供指导。
