Dong Yinping, Wang Wei, Jiang Tao, Xu Jin, Li Menghan, Yan Shaofei, Li Fengqin, Fanning Séamus
Microbiology Laboratory, NHC Key Laboratory of Food Safety Risk Assessment, China National Center for Food Safety Risk Assessment, Beijing, China.
UCD-Centre for Food Safety, School of Public Health, Physiotherapy and Sports Science, University College Dublin, Dublin, Ireland.
Foodborne Pathog Dis. 2022 Sep;19(9):613-621. doi: 10.1089/fpd.2022.0003. Epub 2022 Jul 27.
The epidemiological investigation and laboratory-based confirmation were performed on samples from a family botulism outbreak in Zhangjiakou, Hebei province, China. Forty-four samples, including 14 samples (leftover food, and swabs taken of both food packaging bags and dishes, and serum and vomitus of the victims) related to outbreak and 30 causative food products after outbreak, were collected and analyzed. Isolation, bacterial identification, toxin detection, and whole-genome sequencing of spp. cultured from the latter samples and animal assays were performed. Mice injected with the cultures of the leftover chili chicken feet, together with the inner layer of its packaging bag, the plate for serving it, and supernatant of two patients' serum that demonstrated the typical signs of botulism. The polyvalent anti-botulinum neurotoxin (BoNT) and the monovalent anti-BoNT/E exhibited protective effects when administered to mice. Three cultures were obtained and verified to be positive for BoNT/E. The whole genome analysis of the isolates revealed that the classic gene cluster was found to be located on the chromosomes of all three isolates. Single nucleotide polymorphism analysis suggested that these might be from the same source. Our findings indicated that this botulism outbreak occurred following the ingestion of vacuum-packed chili chicken feet contaminated with BoNT/E produced by
对中国河北省张家口市一起家庭肉毒中毒暴发事件的样本进行了流行病学调查和实验室确诊。共采集并分析了44份样本，其中包括14份与暴发事件相关的样本（剩余食物、食品包装袋和餐具拭子、受害者血清和呕吐物）以及30份暴发事件后的致病食品。对后一组样本培养的菌株进行了分离、细菌鉴定、毒素检测、全基因组测序及动物试验。给小鼠注射剩余辣鸡爪及其包装袋内层、盛放辣鸡爪的盘子的培养物，以及两名表现出典型肉毒中毒症状患者血清的上清液。多价抗肉毒杆菌神经毒素（BoNT）和单价抗BoNT/E对小鼠具有保护作用。获得了3株培养物，经证实BoNT/E呈阳性。对分离株的全基因组分析表明，所有3株分离株的染色体上均发现了经典的BoNT基因簇。单核苷酸多态性分析表明，这些分离株可能来自同一来源。我们的研究结果表明，此次肉毒中毒暴发是由于食用了被BoNT/E污染的真空包装辣鸡爪所致。
