Wang Li-Min, Liu Zhi-Hua, Ren Hong-Lei, Chen Xue-Mei, Wang Jun-Min, Cai Hui-Min, Wei Li-Ping, Tian Hui-Hong, Wang Jian, Wang Li-Juan
The Second School of Clinical Medicine, Southern Medical University; Department of Neurology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangdong Neuroscience Institute, Guangzhou, Guangdong Province, China.
School of Medicine, South China University of Technology, Guangzhou, Guangdong Province, China.
Neural Regen Res. 2023 Feb;18(2):344-349. doi: 10.4103/1673-5374.346459.
The current animal models of stroke primarily model a single intracerebral hemorrhage (ICH) attack, and there is a lack of a reliable model of recurrent ICH. In this study, we established 16-month-old C57BL/6 male mouse models of ICH by injecting collagenase VII-S into the left striatum. Twenty-one days later, we injected collagenase VII-S into the right striatum to simulate recurrent ICH. Our results showed that mice subjected to bilateral striatal hemorrhage had poorer neurological function at the early stage of hemorrhage, delayed recovery in locomotor function, motor coordination, and movement speed, and more obvious emotional and cognitive dysfunction than mice subjected to unilateral striatal hemorrhage. These findings indicate that mouse models of bilateral striatal hemorrhage can well simulate clinically common recurrent ICH. These models should be used as a novel tool for investigating the pathogenesis and treatment targets of recurrent ICH.
目前的中风动物模型主要模拟单次脑出血（ICH）发作，缺乏可靠的复发性ICH模型。在本研究中，我们通过向左纹状体注射VII-S型胶原酶建立了16月龄C57BL/6雄性小鼠ICH模型。21天后，我们向右纹状体注射VII-S型胶原酶以模拟复发性ICH。我们的结果表明，与单侧纹状体出血的小鼠相比，双侧纹状体出血的小鼠在出血早期神经功能较差，运动功能、运动协调性和运动速度恢复延迟，情绪和认知功能障碍更明显。这些发现表明，双侧纹状体出血小鼠模型可以很好地模拟临床上常见的复发性ICH。这些模型应用作研究复发性ICH发病机制和治疗靶点的新型工具。
