Department of Blood Transfusion, The Affiliated Hospital of Qingdao University, Qingdao, 266042, People's Republic of China.
Clinical Medicine Department, Medical College, Qingdao University, Qingdao, 266071, People's Republic of China.
Mikrochim Acta. 2022 Jul 28;189(8):297. doi: 10.1007/s00604-022-05387-2.
An ultrasensitive luminol electrochemiluminescence (ECL) immunosensor was constructed for the detection of prostate specific antigen (PSA) using glucose oxidase-decorated hemin-graphene oxide-gold nanoflowers ternary nanocomposites as probes. Graphene oxide was first modified with hemin and then with gold nanoflowers through an in situ growth method, which has significantly boosted the catalytic activity of this graphene oxide-based peroxidase mimetics. The biocatalytical activity of this ECL immunosensor was thoroughly investigated to achieve selective recognition of the analyte molecules (PSA) by specific binding between antigens and antibodies. The limit of detection was calculated to be 0.32 pg mL with a signal-to-noise ratio of 3. A broad linear range from 7.5 × 10 to 2.5 ng mL was obtained. Such step-by-step assembled biosensor showed controlled nanostructure and exhibited promising application in analysis of human serum samples with a recovery range of 90.6-111.8% and a RSD range of 3.9-5.5%.
一种超灵敏的鲁米诺电化学发光（ECL）免疫传感器，使用葡萄糖氧化酶修饰的血红素-氧化石墨烯-金纳米花三元纳米复合材料作为探针，用于检测前列腺特异性抗原（PSA）。首先通过原位生长法将氧化石墨烯修饰血红素，然后修饰金纳米花，这大大提高了基于氧化石墨烯的过氧化物模拟酶的催化活性。通过彻底研究这种 ECL 免疫传感器的生物催化活性，实现了通过抗原和抗体之间的特异性结合对分析物分子（PSA）进行选择性识别。通过信噪比为 3 计算出的检测限为 0.32 pg mL。获得了从 7.5×10 到 2.5 ng mL 的宽线性范围。这种逐步组装的生物传感器显示出可控的纳米结构，并在分析人血清样品中表现出有前途的应用，回收率范围为 90.6-111.8%，RSD 范围为 3.9-5.5%。
