Institute of Biomedical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan.
J Mater Chem B. 2020 Apr 29;8(16):3192-3212. doi: 10.1039/c9tb02578b.
Ultrasensitive and highly accurate bioassays are critically required for the early detection of various biomarkers and diagnosis of cancer. Electrogenerated chemiluminescence (ECL) is one such technique which shows powerful analytical ability by incorporating ECL active species for sensitive detection of targets. In this regard, the development of ECL as an assay technique is constantly being pushed for better performance and lower detection limits. Incorporation of sensitive immunosensing and aptasensing methods with ECL has the ability to multiply the advantages several-fold. The recent progress in and methods utilized for the enhancement and amplification of ECL detection techniques based on highly sensitive immunosensors and aptasensors have been discussed in this review with regard to widely popular techniques.
超灵敏和高度准确的生物分析对于各种生物标志物的早期检测和癌症的诊断至关重要。电致化学发光（ECL）就是这样一种技术，它通过结合 ECL 活性物质来实现对目标的敏感检测，从而显示出强大的分析能力。在这方面，不断推动 ECL 作为分析技术的发展，以实现更好的性能和更低的检测限。将敏感的免疫传感和适体传感方法与 ECL 结合使用，具有使优势成倍增加的能力。本文综述了基于高灵敏度免疫传感器和适体传感器的 ECL 检测技术的增强和放大方法，讨论了广泛流行的技术。
