Department of Poultry Science, Faculty of Agriculture, Tarbiat Modares University, Nasr, Tehran 14115-336, Iran.
Department of Poultry Science, Faculty of Agriculture, Tarbiat Modares University, Nasr, Tehran 14115-336, Iran.
Poult Sci. 2022 Sep;101(9):102014. doi: 10.1016/j.psj.2022.102014. Epub 2022 Jun 20.
Genetic selection based on the high growth rate and consequently high slaughter weight in broiler chickens has caused many problems in broiler breeders. A negative correlation between growth and reproductive traits has declined semen quality and fertility in roosters. The present study aimed to evaluate the effects of camphor levels on some reproductive parameters included semen parameters, antioxidant status, and testicular development in broiler breeder roosters. Thirty-five ROSS 308 broiler breeder roosters were divided into 5 groups to receive camphor (C) levels: C0, C50, C250, C750, and C1000 ppm for 12 consecutive weeks (31-43 wk). Body weight, seminal volume, sperm concentration, and percentage of live and morphologically normal sperm were not affected by diets (P > 0.05), however, significantly were changed by bird age over the experiment (P < 0.05). Semen quality factor (SQF) significantly was affected by both diets and age (P < 0.05). Mitochondrial activity, apoptotic-like changes, and DNA fragmentation were improved in the groups fed camphor levels compared to the control group (P < 0.05). Testes weight (left, right, and combined weights) and gonadosomatic index were increased linearly by the camphor supplementation (P < 0.05). The serum activity of glutathione peroxidase (GP) was not affected by treatments, however, superoxide dismutase (SOD) activity, ferric ion reducing antioxidant power (FRAP), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity were significantly higher in C50, C250, and C750, respectively (P < 0.05). The lower malondialdehyde (MDA) content in the testes and liver samples was observed in C750 (P < 0.05). Excluding the number of Sertoli cells and blood vessels, other histomorphological traits of testes showed one of the linear or quadratic responses to the camphor levels (P < 0.05). It can be concluded that camphor as an antioxidant source may improve reproduction performance in roosters.
基于肉鸡生长速度快和屠宰体重高的遗传选择给肉鸡饲养者带来了许多问题。生长和繁殖性状之间的负相关降低了公鸡的精液质量和受精能力。本研究旨在评估樟脑水平对一些繁殖参数的影响，包括精液参数、抗氧化状态和种公鸡的睾丸发育。35 只 ROSS 308 种公鸡被分为 5 组，分别接受樟脑（C）水平：C0、C50、C250、C750 和 C1000ppm，连续 12 周（31-43 周）。日粮对体重、精液量、精子浓度和活精子及形态正常精子的比例没有影响（P>0.05），但随着试验中鸟类年龄的变化而显著变化（P<0.05）。精液质量因子（SQF）受日粮和年龄的显著影响（P<0.05）。与对照组相比，喂食樟脑水平的各组中线粒体活性、凋亡样变化和 DNA 片段化得到改善（P<0.05）。睾丸重量（左、右和合并重量）和性腺指数随着樟脑的补充呈线性增加（P<0.05）。血清谷胱甘肽过氧化物酶（GP）的活性不受处理的影响，然而，超氧化物歧化酶（SOD）活性、铁离子还原抗氧化能力（FRAP）和 2,2-二苯基-1-苦基肼（DPPH）自由基清除活性在 C50、C250 和 C750 中分别显著提高（P<0.05）。C750 中观察到睾丸和肝脏样本中的丙二醛（MDA）含量较低（P<0.05）。除了支持细胞和血管的数量外，睾丸的其他组织形态学特征表现出线性或二次响应樟脑水平（P<0.05）。可以得出结论，樟脑作为一种抗氧化剂来源，可能会提高公鸡的繁殖性能。
