Medical College of Nanchang University, Nanchang, 330006, Jiangxi, China.
Department of Orthopedics, Jiangxi Provincial People's Hospital, 152 Aiguo Road, Nanchang, 330006, Jiangxi, China.
BMC Bioinformatics. 2022 Jul 28;23(1):306. doi: 10.1186/s12859-022-04849-x.
Ewing sarcoma (ES) is the second most common primary malignant bone tumor mainly occurring in children, adolescents and young adults with high metastasis and mortality. Autophagy has been reported to be involved in the survival of ES, but the role remains unclear. Therefore, it's necessary to investigate the prognostic value of autophagy related genes using bioinformatics methods.
ATG2B, ATG10 and DAPK1 were final screened genes for a prognostic model. KM and risk score plots showed patients in high score group had better prognoses both in training and validation sets. C-indexes of the model for training and validation sets were 0.68 and 0.71, respectively. Calibration analyses indicated the model had high prediction accuracy in training and validation sets. The AUC values of ROC for 1-, 3-, 5-year prediction were 0.65, 0.73 and 0.84 in training set, 0.88, 0.73 and 0.79 in validation set, which suggested high prediction accuracy of the model. Decision curve analyses showed that patients could benefit much from the model. Differential and functional analyses suggested that autophagy and apoptosis were upregulated in high risk score group.
ATG2B, ATG10 and DAPK1 were autophagy related genes with potential protective function in ES. The prognostic model established by them exhibited excellent prediction accuracy and discriminatory capacities. They might be used as potential prognostic biomarkers and therapeutic targets in ES.
尤文肉瘤（ES）是第二常见的原发性骨恶性肿瘤，主要发生在儿童、青少年和年轻成人，具有高转移和高死亡率。自噬已被报道参与 ES 的存活，但作用仍不清楚。因此，有必要使用生物信息学方法研究自噬相关基因的预后价值。
最终筛选出 ATG2B、ATG10 和 DAPK1 作为预后模型的基因。KM 和风险评分图显示，高评分组患者在训练集和验证集中的预后均较好。模型在训练集和验证集中的 C 指数分别为 0.68 和 0.71。校准分析表明该模型在训练集和验证集中具有较高的预测准确性。ROC 的 AUC 值在训练集中分别为 1、3、5 年预测的 0.65、0.73 和 0.84，在验证集中的 AUC 值分别为 0.88、0.73 和 0.79，这表明该模型具有较高的预测准确性。决策曲线分析表明，该模型对患者具有很大的受益。差异和功能分析表明，自噬和细胞凋亡在高风险评分组中上调。
ATG2B、ATG10 和 DAPK1 是 ES 中具有潜在保护功能的自噬相关基因。由它们建立的预后模型表现出优异的预测准确性和区分能力。它们可能成为 ES 中潜在的预后生物标志物和治疗靶点。
