使用标准化翻译起始区域的信号放大。 （原句“Signal amplification of using...”表述有误，推测正确表述应是“Signal amplification by using...” ，这里按推测后的正确意思翻译）
Signal amplification of using a standardized translation initiation region.
作者信息
Shilling Patrick J, Khananisho Diana, Cumming Alister J, Söderström Bill, Daley Daniel O
机构信息
Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden.
Australian Institute for Microbiology and Infection, University of Technology Sydney, Sydney, NSW, Australia.
出版信息
Synth Biol (Oxf). 2022 Jul 5;7(1):ysac009. doi: 10.1093/synbio/ysac009. eCollection 2022.
is a genetic fragment that regulates the expression of the operon in bacteria, which is required for the metabolism of L-arabinose. It is widely used in bioengineering applications because it can drive regulatable and titratable expression of genes and genetic pathways in microbial cell factories. A notable limitation of is that it generates a low signal when induced with high concentrations of L-arabinose (the maximum ON state). Herein we have amplified the maximum ON state of by coupling it to a synthetically evolved translation initiation region ( ). The coupling maintains regulatable and titratable expression from and yet increases the maximal ON state by >5-fold. The general principle demonstrated in the study can be applied to amplify the signal from similar genetic modules. Graphical Abstract.
是一个调节细菌操纵子表达的基因片段，这是L-阿拉伯糖代谢所必需的。它在生物工程应用中被广泛使用，因为它可以驱动微生物细胞工厂中基因和遗传途径的可调节和可滴定表达。的一个显著局限性是，当用高浓度L-阿拉伯糖诱导时（最大开启状态），它产生的信号较低。在此，我们通过将其与一个经过合成进化的翻译起始区域（）偶联，增强了的最大开启状态。这种偶联保持了来自的可调节和可滴定表达，但将最大开启状态提高了>5倍。该研究中展示的一般原理可用于增强来自类似遗传模块的信号。图形摘要。
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