基因敲入能够在整个生命周期中监测线粒体形态。
knock-in enables the monitoring of mitochondrial morphology throughout lifespan.
作者信息
Begelman David V, Woods Georgia, Bhaumik Dipa, Angeli Suzanne, Foulger Anna C, Lucanic Mark, Lan Jianfeng, Andersen Julie K, Lithgow Gordon J
机构信息
The Buck Institute for Research on Aging.
University of Maine, Molecular & Biomedical Sciences.
出版信息
MicroPubl Biol. 2022 Jul 17;2022. doi: 10.17912/micropub.biology.000599. eCollection 2022.
We used CRISPR/Cas9 gene editing in in order to fluorescently tag endogenous aconitase-2 (ACO-2). ACO-2 is a mitochondrially localized protein, and the strain enabled the examination of native mitochondrial morphology in live animals. Here we validate that the strain displays the prototypic changes in mitochondrial morphology known to occur during aging and upon paraquat (PQ) induced mitochondrial stress. We also provide evidence that the ACO-2::GFP reporter can serve as a superior means for tracking mitochondrial morphology than conventional MitoTracker dyes-especially in aged-worms.
为了对内源乌头酸酶-2（ACO-2）进行荧光标记，我们使用了CRISPR/Cas9基因编辑技术。ACO-2是一种定位于线粒体的蛋白质，该品系能够在活体动物中检测天然线粒体形态。在此，我们验证了该品系呈现出在衰老过程中以及百草枯（PQ）诱导的线粒体应激时已知会发生的线粒体形态的典型变化。我们还提供证据表明，与传统的线粒体追踪染料相比，ACO-2::GFP报告基因可作为一种更优的追踪线粒体形态的手段——尤其是在衰老蠕虫中。
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