Lyrenäs S, Nyberg F, Willdeck-Lund G, Lindström L, Lindberg B, Terenius L
Ups J Med Sci. 1987;92(1):37-45. doi: 10.3109/03009738709178676.
Opioid activity in cerebrospinal fluid (CSF) was estimated by radioreceptor-assay (RRA) in samples obtained from ten women at term pregnancy and in the early puerperal period. The samples were fractioned on Sephadex G-10 columns and two opioid receptor active fractions, FI and FII, were recovered. Two pools of FII materials from pregnant and puerperal women, respectively, were further analyzed by electrophoresis and the concentrations of opioid activity were measured by radioreceptor assay. There was a significant rise in receptor-assayed FII opioid activity in late pregnancy as well as in the early puerperal period compared to that of healthy, nonpregnant, nonpuerperal females. Pooled FII material obtained before delivery could be separated into two major components tentatively assigned the hexapeptide [Met]enkephalin-Lys6 and the heptapeptide [Met]enkephalin-Arg6-Phe7. These two opioid peptides both have their origin in the [Met]enkephalin precursor, proenkephalin A. In the puerperal period there was predominance of only one of these components.
通过放射受体分析法（RRA）对10名足月妊娠和产后早期女性的脑脊液（CSF）样本中的阿片类活性进行了评估。样本在葡聚糖G - 10柱上进行分离，回收了两个阿片受体活性组分，即FI和FII。分别对来自孕妇和产妇的两池FII物质进行了进一步的电泳分析，并通过放射受体分析法测量了阿片类活性的浓度。与健康、未怀孕、未产后的女性相比，妊娠晚期和产后早期受体检测的FII阿片类活性显著升高。分娩前获得的合并FII物质可初步分离为两个主要成分，分别为六肽[Met]脑啡肽 - Lys6和七肽[Met]脑啡肽 - Arg6 - Phe7。这两种阿片肽均起源于[Met]脑啡肽前体，即前脑啡肽A。在产后期，这些成分中只有一种占主导地位。
