Department of Chemistry "Ugo Schiff" and CSGI, University of Florence, Sesto Fiorentino, Florence I-50019, Italy.
Brussels Innovation Centre, Temselaan 100, Strombeek-bever B-1853, Belgium.
ACS Appl Bio Mater. 2022 Aug 15;5(8):3703-3712. doi: 10.1021/acsabm.2c00218. Epub 2022 Jul 29.
Usually, to characterize bacterial cells' susceptibility to antimicrobials, basic microbiology techniques such as serial dilutions or disk assays are used. In this work, we present an approach focused on combining static light scattering (SLS) and ultra-/small angle X-ray scattering (USAXS/SAXS). This approach was used to support microbiology techniques, with the aim of understanding the structural changes caused to bacteria when they are exposed to different stresses like pH, oxidation, and surfactants. Using USAXS/SAXS and SLS data, we developed a detailed multiscale model for a Gram-positive bacterium, , and we extracted information regarding changes in the overall size and cell thickness induced by different stresses (i.e., pH and hydrogen peroxide). Increasing the concentration of hydrogen peroxide leads to a progressive reduction in cell wall thickness. Moreover, the concomitant use of pH and hydrogen peroxide provides evidence for a synergy in inhibiting the growth. These promising results will be used as a starting base to further investigate more complex formulations and improve/refine the data modeling of bacteria in the small angle scattering regime.
通常，为了表征细菌细胞对抗菌药物的敏感性，会使用基础微生物学技术，如连续稀释或圆盘测定法。在这项工作中，我们提出了一种专注于结合静态光散射（SLS）和超/小角 X 射线散射（USAXS/SAXS）的方法。该方法用于支持微生物学技术，旨在了解当细菌暴露于不同的应激源（如 pH 值、氧化和表面活性剂）时，细菌结构所发生的变化。我们使用 USAXS/SAXS 和 SLS 数据，为革兰氏阳性菌 开发了一个详细的多尺度模型，并提取了由不同应激源（即 pH 值和过氧化氢）引起的整体大小和细胞厚度变化的信息。过氧化氢浓度的增加导致细胞壁厚度的逐渐减小。此外，同时使用 pH 值和过氧化氢为抑制 生长的协同作用提供了证据。这些有希望的结果将被用作进一步研究更复杂配方和改进/细化小角散射范围内细菌数据建模的起点。
