天然药物产生的活性氧的测定及其抗菌活性

Tajima Noriko, Takasaki Makiko, Fukamachi Haruka, Igarashi Takeshi, Nakajima Yoshijiro, Arakawa Hidetoshi

Analytical Chemistry, School of Pharmacy, Showa Univ., 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.

Oral Microbiology and Immunology, School of Dentistry, Showa Univ., 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.

J Pharm Anal. 2016 Aug;6(4):214-218. doi: 10.1016/j.jpha.2016.04.003. Epub 2016 Apr 7.

Extracts of 16 natural medicine powders (, and ) were assayed for reactive oxygen concentrations using the peroxyoxalate chemiluminescent detection system. High luminescence intensity was observed in , and . Additional experiments identified the reactive oxygen species as hydrogen peroxide. generated 2.4×10 mol/L hydrogen peroxide from a 1 mg/mL solution. In bacterial growth tests, extract had antibacterial activity against , , , , , , and . This antibacterial activity was decreased by the addition of catalase. It revealed that hydrogen peroxide which produced participated in antibacterial activity.

使用过氧草酸酯化学发光检测系统对16种天然药粉提取物（、和）的活性氧浓度进行了测定。在、和中观察到高发光强度。进一步实验确定活性氧种类为过氧化氢。从1mg/mL溶液中产生2.4×10⁻⁶mol/L过氧化氢。在细菌生长试验中，提取物对、、、、、和具有抗菌活性。添加过氧化氢酶后，这种抗菌活性降低。这表明产生的过氧化氢参与了抗菌活性。