Su Lijie, Zeng Yupeng, Li Guokun, Chen Jing, Chen Xiaoyi
Department of Nutrition and Food Hygiene, School of Public Health, Guangzhou Medical University, Guangzhou, China.
Sino-French Hoffmann Institute, School of Basic Medical Science, Guangzhou Medical University, Guangzhou, China.
Phytother Res. 2022 Dec;36(12):4558-4572. doi: 10.1002/ptr.7575. Epub 2022 Jul 29.
High-fat diet-induced obesity is characterized by low-grade inflammation, which has been linked to gut microbiota dysbiosis. We hypothesized that quercetin supplementation would alter gut microbiota and reduce inflammation in obese mice. Male C57BL/6J mice, 4 weeks of age, were divided into 3 groups, including a low-fat diet group, a high-fat diet (HFD) group, and a high-fat diet plus quercetin (HFD+Q) group. The mice in HFD+Q group were given 50 mg per kg BW quercetin by gavage for 20 weeks. The body weight, fat accumulation, gut barrier function, glucose tolerance, and adipose tissue inflammation were determined in mice. 16 s rRNA amplicon sequence and non-targeted metabolomics analysis were used to explore the alteration of gut microbiota and metabolites. We found that quercetin significantly alleviated HFD-induced obesity, improved glucose tolerance, recovered gut barrier function, and reduced adipose tissue inflammation. Moreover, quercetin ameliorated HFD-induced gut microbiota disorder by regulating the abundance of gut microbiota, such as Adlercreutzia, Allobaculum, Coprococcus_1, Lactococcus, and Akkermansia. Quercetin influenced the production of metabolites that were linked to alterations in obesity-related inflammation and oxidative stress, such as Glycerophospho-N-palmitoyl ethanolamine, sanguisorbic acid dilactone, O-Phospho-L-serine, and P-benzoquinone. Our results demonstrate that the anti-obesity effects of quercetin may be mediated through regulation in gut microbiota and metabolites.
高脂饮食诱导的肥胖症的特征是低度炎症，这与肠道微生物群失调有关。我们假设补充槲皮素会改变肠道微生物群并减轻肥胖小鼠的炎症。将4周龄的雄性C57BL/6J小鼠分为3组，包括低脂饮食组、高脂饮食（HFD）组和高脂饮食加槲皮素（HFD+Q）组。HFD+Q组的小鼠通过灌胃给予每千克体重50毫克槲皮素，持续20周。测定小鼠的体重、脂肪堆积、肠道屏障功能、葡萄糖耐量和脂肪组织炎症。使用16S rRNA扩增子序列和非靶向代谢组学分析来探索肠道微生物群和代谢物的变化。我们发现槲皮素显著减轻了HFD诱导的肥胖症，改善了葡萄糖耐量，恢复了肠道屏障功能，并减轻了脂肪组织炎症。此外，槲皮素通过调节肠道微生物群的丰度，如阿德勒克雷茨菌属、别氏杆菌属、粪球菌属1、乳球菌属和阿克曼氏菌属，改善了HFD诱导的肠道微生物群紊乱。槲皮素影响了与肥胖相关炎症和氧化应激变化相关的代谢物的产生，如甘油磷酸-N-棕榈酰乙醇胺、山梨酸双内酯、O-磷酸-L-丝氨酸和对苯醌。我们的结果表明，槲皮素的抗肥胖作用可能是通过调节肠道微生物群和代谢物来介导的。
