Menevse Esma, Dursunoglu Duygu, Cetin Nihal, Korucu Emine Nedime, Erbayram Fatma Zehra
Department of Biochemistry, Faculty of Medicine, Selcuk University, Konya, Turkey.
Department of Histology & Embryology, Faculty of Medicine, Selcuk University, Konya, Turkey.
Rev Int Androl. 2022 Oct-Dec;20(4):266-273. doi: 10.1016/j.androl.2021.05.002. Epub 2022 Jul 26.
Around 15% of productive couples in the world are infertile. Recent years, biochemical mechanisms leads to male infertility are started to research. Redox regulation and oxidative stress (OS) show importance in the pathogenesis of infertility in male. Malondialdehyde (MDA) and Glutathione (GSH) are biochemical indicatives of sperm damage by reactive oxygen species (ROS). In addition, sperm are coated with a thick glycocalyx rich in sialic acids. It is aimed to determine and evaluate the differences between normozoospermic and oligozoospermic individuals according to sialic acid, MDA and GSH concentrations and correlations between spermyogram and these parameters.
This study was carried out on seminal plasma of individuals who admitted to Selcuk University Faculty of Medicine IVF Unit Andrology Laboratory. The groups were divided into two as normozoospermics (n=30, sperm concentration≥15million/mL), and oligozoospermics (n=30, sperm concentration<15million/mL). Spermyogram were evaluated regarding WHO (2010) Kruger criteria. GSH, MDA and sialic acid concentrations were analyzed in seminal plasma. Diagnostic performance of sialic acid has been determined with ROC curve analysis.
Sialic acid levels were significantly lower in Normozoospermic than Oligozoospermic individuals (p<0.0001), MDA and GSH levels were not differ significantly in both groups (p>0.05). Sialic acid correlated significantly with most of the spermyogram findings. When diagnostic performance of sialic acid was evaluated, the cut off value of sialic acid found as 4.175nmol/mL by ROC curve.
High seminal plasma sialic acid levels may be used as a biomarker and sialic acid is important determinant in oligozoospermia.
全球约15%的育龄夫妇存在不孕问题。近年来，引发男性不育的生化机制开始受到研究。氧化还原调节和氧化应激（OS）在男性不育的发病机制中显示出重要性。丙二醛（MDA）和谷胱甘肽（GSH）是活性氧（ROS）对精子造成损伤的生化指标。此外，精子表面覆盖着一层富含唾液酸的厚糖萼。本研究旨在根据唾液酸、MDA和GSH浓度，以及精子图谱与这些参数之间的相关性，确定并评估正常精子数个体与少精子症个体之间的差异。
本研究针对塞尔丘克大学医学院体外受精科男科实验室收治个体的精液进行。研究对象分为两组，即正常精子数组（n = 30，精子浓度≥1500万/mL）和少精子症组（n = 30，精子浓度<1500万/mL）。根据世界卫生组织（2010年）克鲁格标准评估精子图谱。分析精液中GSH、MDA和唾液酸的浓度。通过ROC曲线分析确定唾液酸的诊断性能。
正常精子数个体的唾液酸水平显著低于少精子症个体（p<0.0001），两组的MDA和GSH水平无显著差异（p>0.05）。唾液酸与大多数精子图谱结果显著相关。在评估唾液酸的诊断性能时，ROC曲线得出唾液酸的截断值为4.175nmol/mL。
精液中高唾液酸水平可作为生物标志物，唾液酸是少精子症的重要决定因素。
