Yin Tao, Yue Xinyu, Li Qian, Zhou Xinyu, Dong Rui, Chen Jiayi, Zhang Runtao, Wang Xin, He Shitao, Jiang Tingting, Tao Fangbiao, Cao Yunxia, Ji Dongmei, Liang Chunmei
Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei, 230022, Anhui, China.
Department of Health Inspection and Quarantine, School of Public Health, Anhui Medical University, No. 81 Meishan Road, Hefei, 230032, Anhui, China.
Biol Trace Elem Res. 2024 Jul;202(7):2941-2953. doi: 10.1007/s12011-023-03888-6. Epub 2023 Oct 7.
Epidemiological studies on the associations between the levels of oxidative stress (OS) indicators (MDA, SOD, and GSH) in seminal plasma and the risk of idiopathic oligo-asthenotera-tozoospermia (OAT) are still inconsistent. Additionally, whether the associations can be altered by the status of essential trace elements is still unknown. To investigate the relationship between MDA, SOD, and GSH levels in seminal plasma and the risk of idiopathic OAT, and further to examine whether levels of iron (Fe), copper (Cu), and selenium (Se) in seminal plasma can alter the associations. A total of 148 subjects (75 idiopathic OAT cases and 73 controls) were included in this study. Seminal plasma samples from all the participants were measured for levels of MDA, SOD, GSH, Fe, Cu, and Se. Unconditional logistic regression models were used to examine the associations between three oxidative stress indicators and the risk of idiopathic OAT. Bayesian kernel machine regression was performed to determine the joint effects of levels of three OS indicators on the risk of idiopathic OAT. Subgroup analyses were performed to explore whether the above associations can be different when Fe, Cu, and Se were in different levels. The level of MDA in seminal plasma was positively associated with the risk of idiopathic OAT, with adjusted odds ratio (OR) and 95% confidence interval (CI) of 2.38 (1.17, 4.83), and SOD and GSH levels were not associated with the risk of idiopathic OAT. In BKMR analyses, we found a significant positive association between the mixture of MDA, SOD, and GSH levels and the risk of idiopathic OAT at concentrations below the 65th percentile, while a negative association at concentrations above it. In subgroup analysis, a positive association was observed between MDA levels in seminal plasma and the risk of idiopathic OAT in the high-Cu group (adjusted OR = 3.66, 95%CI = 1.16, 11.57), while no significant association was found in the low-Cu group (adjusted OR = 1.43, 95%CI = 0.44, 4.58). Additionally, a negative association was found between GSH levels in seminal plasma and the risk of idiopathic OAT in the high-Se group (adjusted OR = 0.34, 95%CI = 0.11, 0.99), while no significant association was observed in the low-Se group (adjusted OR = 1.96, 95%CI = 0.46, 8.27). The levels of MDA, SOD, and GSH in seminal plasma were associated with the risk of idiopathic OAT, and the levels of Cu and Se in seminal plasma may alter the associations.
关于精浆中氧化应激（OS）指标（丙二醛、超氧化物歧化酶和谷胱甘肽）水平与特发性少弱畸精子症（OAT）风险之间关联的流行病学研究结果仍不一致。此外，这些关联是否会因必需微量元素的状态而改变尚不清楚。为了研究精浆中丙二醛、超氧化物歧化酶和谷胱甘肽水平与特发性OAT风险之间的关系，并进一步探讨精浆中铁、铜和硒的水平是否会改变这些关联。本研究共纳入148名受试者（75例特发性OAT病例和73名对照）。检测了所有参与者精浆样本中丙二醛、超氧化物歧化酶、谷胱甘肽、铁、铜和硒的水平。使用无条件逻辑回归模型来检验三种氧化应激指标与特发性OAT风险之间的关联。进行贝叶斯核机器回归以确定三种OS指标水平对特发性OAT风险的联合效应。进行亚组分析以探讨当铁、铜和硒处于不同水平时上述关联是否会有所不同。精浆中丙二醛水平与特发性OAT风险呈正相关，调整后的比值比（OR）和95%置信区间（CI）为2.38（1.17，4.83），而超氧化物歧化酶和谷胱甘肽水平与特发性OAT风险无关。在贝叶斯核机器回归分析中，我们发现丙二醛、超氧化物歧化酶和谷胱甘肽水平的混合物与特发性OAT风险在浓度低于第65百分位数时有显著正相关，而在浓度高于该百分位数时呈负相关。在亚组分析中，高铜组精浆中丙二醛水平与特发性OAT风险呈正相关（调整后的OR = 3.66，95%CI = 1.16，11.57），而低铜组未发现显著关联（调整后的OR = 1.43，95%CI = 0.44，4.58）。此外，高硒组精浆中谷胱甘肽水平与特发性OAT风险呈负相关（调整后的OR = 0.34，95%CI = 0.11，0.99），而低硒组未观察到显著关联（调整后的OR = 1.96，95%CI = 0.46，8.27）。精浆中丙二醛、超氧化物歧化酶和谷胱甘肽水平与特发性OAT风险相关，精浆中铜和硒的水平可能会改变这些关联。
