Freitas R G B O N, Vasques A C J, Fernandes G R, Ribeiro F B, Solar I, Barbosa M G, Almeida-Pititto B, Geloneze B, Ferreira S R G
Department of Epidemiology, School of Public Health, University of São Paulo, São Paulo, Brazil.
Laboratory of Investigation in Metabolism and Diabetes, Gastrocentro, School of Medical Sciences - University of Campinas, São Paulo, Brazil.
Eur J Clin Nutr. 2022 Dec;76(12):1705-1712. doi: 10.1038/s41430-022-01182-7. Epub 2022 Jul 29.
Intrauterine environment can influence the offspring's body adiposity whose distribution affect the cardiometabolic risk. Underlying mechanisms may involve the gut microbiome. We investigated associations of gestational weight gain with the adult offspring's gut microbiota, body adiposity and related parameters in participants of the Nutritionists' Health Study.
This cross-sectional analysis included 114 women who had early life and clinical data, body composition, and biological samples collected. The structure of fecal microbiota was analyzed targeting the V4 region of the 16 S rRNA gene. Beta diversity was calculated by PCoA and PERMANOVA used to test the impact of categorical variables into the diversity. Bacterial clusters were identified based on the Jensen-Shannon divergence matrix and Calinski-Harabasz index. Correlations were tested by Spearman coefficient.
Median age was 28 (IQR 24-31) years and BMI 24.5 (IQR 21.4-28.0) kg/m. Fifty-eight participants were assigned to a profile driven by Prevotella and 56 to another driven by Blautia. Visceral adipose tissue was correlated to abundance of Acidaminococcus genus considering the entire sample (r = 0.37; p < 0.001) and the profiles (Blautia: r = 0.35, p = 0.009, and Prevotella: r = 0.38, p = 0.006). In Blautia-driven profile, the same genus was also correlated to maternal gestational weight gain (r = 0.38, p = 0.006).
Association of Acidaminococcus with gestational weight gain could reinforce the relevance with mothers' nutritional status for gut colonization at the beginning of life. Whether Acidaminococcus abundance could be a marker for central distribution of adiposity in young women requires further investigation.
子宫内环境会影响后代的身体肥胖程度，而肥胖的分布会影响心脏代谢风险。潜在机制可能涉及肠道微生物群。我们在营养学家健康研究的参与者中，调查了孕期体重增加与成年后代肠道微生物群、身体肥胖及相关参数之间的关联。
这项横断面分析纳入了114名具有早年生活和临床数据、身体成分及生物样本的女性。针对16S rRNA基因的V4区域分析粪便微生物群的结构。通过主坐标分析（PCoA）计算β多样性，并使用PERMANOVA检验分类变量对多样性的影响。基于詹森 - 香农散度矩阵和卡林斯基 - 哈拉巴斯指数识别细菌簇。通过斯皮尔曼系数检验相关性。
中位年龄为28（四分位间距24 - 31）岁，体重指数为24.5（四分位间距21.4 - 28.0）kg/m²。58名参与者被归为以普雷沃氏菌为主导的类型，56名被归为以布劳特氏菌为主导的另一种类型。考虑整个样本时，内脏脂肪组织与氨基酸球菌属的丰度相关（r = 0.37；p < 0.001），在不同类型中（布劳特氏菌主导型：r = 0.35，p = 0.009，普雷沃氏菌主导型：r = 0.38，p = 0.006）也是如此。在布劳特氏菌主导的类型中，同一属也与母亲孕期体重增加相关（r = 0.38，p = 0.006）。
氨基酸球菌与孕期体重增加的关联可能会加强其与母亲营养状况在生命早期肠道定植方面的相关性。氨基酸球菌丰度是否可作为年轻女性肥胖中心分布的标志物，还需要进一步研究。
