Department of Environmental Science and Engineering, Ewha Womans University, Seoul, 03760, Republic of Korea.
Sci Rep. 2022 Jul 29;12(1):13018. doi: 10.1038/s41598-022-17356-z.
The demand for rhizoremediation technology that can minimize greenhouse gas emissions while effectively removing pollutants in order to mitigate climate change has increased. The inoculation effect of NO-reducing Pseudomonas sp. TF716 on NO emissions and on remediation performance during the rhizoremediation of diesel-contaminated soil planted with tall fescue (Festuca arundinacea) or maize (Zea mays) was investigated. Pseudomonas sp. TF716 was isolated from the rhizosphere soil of tall fescue. The maximum NO reduction rate of TF716 was 18.9 mmol NO g dry cells h, which is superior to the rates for previously reported Pseudomonas spp. When Pseudomonas sp. TF716 was added to diesel-contaminated soil planted with tall fescue, the soil NO-reduction potential was 2.88 times higher than that of soil with no inoculation during the initial period (0-19 d), and 1.08-1.13 times higher thereafter. However, there was no enhancement in the NO-reduction potential for the soil planted with maize following inoculation with strain TF716. In addition, TF716 inoculation did not significantly affect diesel degradation during rhizoremediation, suggesting that the activity of those microorganisms involved in diesel degradation was unaffected by TF716 treatment. Analysis of the dynamics of the bacterial genera associated with NO reduction showed that Pseudomonas had the highest relative abundance during the rhizoremediation of diesel-contaminated soil planted with tall fescue and treated with strain TF716. Overall, these results suggest that NO emissions during the rhizoremediation of diesel-contaminated soil using tall fescue can be reduced with the addition of Pseudomonas sp. TF716.
为了缓解气候变化，需要一种能够最小化温室气体排放、同时有效去除污染物的根茎修复技术，因此对该技术的需求日益增加。本研究调查了接种具有还原 NO 能力的 Pseudomonas sp. TF716 对污染土壤根茎修复过程中 NO 排放和修复性能的影响，所用污染土壤为种植高羊茅（Festuca arundinacea）或玉米（Zea mays）的柴油污染土壤。Pseudomonas sp. TF716 是从高羊茅根际土壤中分离得到的。TF716 的最大 NO 还原率为 18.9 mmol NO g 干细胞 h，优于先前报道的 Pseudomonas spp。当将 Pseudomonas sp. TF716 接种到种植高羊茅的柴油污染土壤中时，接种初期（0-19 d）土壤的 NO 还原潜力比未接种时高 2.88 倍，此后则高 1.08-1.13 倍。然而，接种 TF716 对种植玉米的土壤的 NO 还原潜力没有增强。此外，TF716 接种对根茎修复过程中柴油的降解没有显著影响，这表明参与柴油降解的微生物的活性不受 TF716 处理的影响。与 NO 还原相关的细菌属的动态分析表明，在接种 Pseudomonas sp. TF716 处理的高羊茅种植的柴油污染土壤根茎修复过程中，Pseudomonas 的相对丰度最高。总的来说，这些结果表明，在接种 Pseudomonas sp. TF716 的高羊茅种植的柴油污染土壤根茎修复过程中，可以减少 NO 的排放。
