Department of Environmental Science and Engineering, Ewha Womans University, Seoul, Republic of Korea.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2020;55(12):1459-1467. doi: 10.1080/10934529.2020.1812321. Epub 2020 Sep 22.
A new nitrous oxide (NO)-reducing bacterium was isolated from a consortium that was enriched using advanced wastewater treatment sludge as an inoculum and NO as the sole nitrogen source. The isolated facultative anaerobe was identified as sp. HJ23. sp. HJ23 exhibited optimum NO-reducing activity with a C/N ratio of 62 at pH 6 in the temperature range of 37 °C to 40 °C. The optimum carbon source for NO reduction was a mixture of glucose and acetate. The maximum rate of NO reduction by sp. HJ23 was 4.8 mmol·g-dry cell·h, and its NO-reducing activity was higher than other known NO reducers. sp. HJ23 possessed several functional genes for denitrification. These included (NO reductase), (NO reductase), (NO reductase), and (NO reductase) genes. These results suggest that sp. HJ23 can be applied in the denitrification process to minimalize NO emission.
从以高级废水处理污泥为接种物和一氧化二氮 (NO) 为唯一氮源的共生物种中分离到一种新型的能还原一氧化二氮的细菌。分离到的兼性厌氧菌被鉴定为 sp. HJ23。 sp. HJ23 在 pH 值为 6、温度范围为 37°C 至 40°C 时，以 C/N 比为 62 时表现出最佳的 NO 还原活性。NO 还原的最佳碳源是葡萄糖和乙酸的混合物。 sp. HJ23 的最大 NO 还原速率为 4.8mmol·g-干细胞·h，其 NO 还原活性高于其他已知的 NO 还原剂。 sp. HJ23 拥有几种用于反硝化的功能基因。这些基因包括 (NO 还原酶)、 (NO 还原酶)、 (NO 还原酶) 和 (NO 还原酶) 基因。这些结果表明， sp. HJ23 可应用于反硝化过程，以最大限度地减少 NO 排放。
