Department of Urology, Graduate School of Medicine, Yamaguchi University, 1‑1‑1, Minamikogushi, Ube, Yamaguchi, 755-8505, Japan.
Division of Anti-Aging Medicine, Center for Molecular Medicine, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke, Tochigi, 329-0498, Japan.
Sci Rep. 2022 Jul 29;12(1):13030. doi: 10.1038/s41598-022-17405-7.
Calciprotein particles (CPPs) are circulating colloidal mineral-protein complexes containing crystalline and/or non-crystalline (amorphous) calcium-phosphate (CaPi). Serum CPP levels correlate with vascular stiffness and calcification in patients with chronic kidney disease (CKD). In vitro studies showed that CPPs containing crystalline CaPi were more arteriosclerogenic and inflammogenic than CPPs without containing crystalline CaPi. Thus, we hypothesized that not only the quantity but also the quality of CPPs (the phase of CaPi) might affect clinical outcomes. To test this hypothesis, we quantified amorphous CaPi ratio defined as the ratio of the amorphous CaPi amount to the total CaPi amount in serum CPPs from 183 hemodialysis patients and explored its possible correlation with serum parameters associated with prognosis of hemodialysis patients. Multivariate analysis revealed that the amorphous CaPi ratio correlated positively with hemoglobin and negatively with fibroblast growth factor-21 (FGF21), which remained significant after adjusting for the total CaPi amount. Because low hemoglobin and high FGF21 are associated with increased mortality, the present study warrants further studies to determine whether low amorphous CaPi ratio in circulating CPPs may be associated with poor prognosis in hemodialysis patients.
钙磷蛋白颗粒 (CPPs) 是循环胶体矿物-蛋白复合物，含有结晶和/或非晶（无定形）的钙磷 (CaPi)。血清 CPP 水平与慢性肾脏病 (CKD) 患者的血管僵硬和钙化相关。体外研究表明，含有结晶 CaPi 的 CPP 比不含结晶 CaPi 的 CPP 更具有动脉粥样硬化和促炎作用。因此，我们假设 CPP 的质量（CaPi 的相）不仅会影响临床结果，而且 CPP 的数量也会影响。为了验证这一假设，我们从 183 名血液透析患者的血清 CPP 中定量了无定形 CaPi 比例（定义为无定形 CaPi 量与总 CaPi 量的比值），并探讨了其与与血液透析患者预后相关的血清参数的可能相关性。多变量分析显示，无定形 CaPi 比例与血红蛋白呈正相关，与成纤维细胞生长因子-21（FGF21）呈负相关，在调整总 CaPi 量后仍具有显著相关性。由于低血红蛋白和高 FGF21 与死亡率增加相关，因此本研究需要进一步研究以确定循环 CPPs 中低无定形 CaPi 比例是否与血液透析患者的预后不良相关。
