Division of Pharmacology, School of Korean Medicine, Pusan National University, Gyeongnam, 50612, South Korea.
Department of Pathology, College of Korean Medicine, Dongshin University, Jeonnam, 58245, South Korea.
Chin J Integr Med. 2022 Aug;28(8):719-724. doi: 10.1007/s11655-022-3517-0. Epub 2022 Jul 30.
To investigate the anti-inflammatory potential of Ampelopsis japonica on contact dermatitis (CD).
A total of 38 Balb/c mice were divided into 5 groups by using a random number table: normal mice (n=6), CD model mice (n=8), CD mice treated with 3 or 30 mg/kg of the ethanol extract of A. japonica (EEAJ, n=8) and 7.5 mg/kg dexamethasone treated CD mice (DEX, n=8). CD was induced using topical application of 1-fluoro-2,4-dinitrofluorobenzene in mice. EEAJ and DEX were topically applied to the shaved skin of each mouse for 6 days, and the effects of EEAJ and DEX on skin lesions and color, histopathological abnormalities such as epidermal hyperplasia and immune cell infiltration, and tumor necrosis factor (TNF)-α, interleukin-6 (IL-6), and monocyte chemoattractant protein-1 (MCP-1) production were investigated. The effects on changes in body weights and spleen/body weight ratio were also investigated.
EEAJ at 30 mg/kg significantly prevented scaling, erythema and enlargement of skin weight compared to using carbon dioxide. EEAJ also prevented epithelial hyperplasia and immune cell infiltrations induced by repeated application of DNFB (P<0.01). In addition, EEAJ significantly lowered levels of TNF-α, IL-6 and MCP-1 (P<0.05 or P<0.01). The anti-inflammatory effects of EEAJ were similar to those of DEX.
A. japonica may be a new therapeutic agent with the potential to reduce or replace corticosteroids and its mechanisms are closely related to regulation of TNF-α production.
研究落葵对接触性皮炎（CD）的抗炎作用。
38 只 Balb/c 小鼠采用随机数字表法分为 5 组：正常小鼠（n=6）、CD 模型小鼠（n=8）、3 或 30mg/kg 落葵乙醇提取物（EEAJ）处理的 CD 小鼠（n=8）和 7.5mg/kg 地塞米松处理的 CD 小鼠（DEX，n=8）。采用 1-氟-2,4-二硝基氟苯对小鼠进行外用诱导 CD。EEAJ 和 DEX 分别涂于每只小鼠剃毛皮肤，连续 6 天，观察 EEAJ 和 DEX 对皮肤病变和颜色、表皮增生和免疫细胞浸润等组织病理学异常、肿瘤坏死因子（TNF）-α、白细胞介素-6（IL-6）和单核细胞趋化蛋白-1（MCP-1）产生的影响，并观察对体重和脾/体重比变化的影响。
与二氧化碳相比，30mg/kg 的 EEAJ 可显著预防脱屑、红斑和皮肤重量增加。EEAJ 还可预防重复应用 DNFB 引起的上皮增生和免疫细胞浸润（P<0.01）。此外，EEAJ 可显著降低 TNF-α、IL-6 和 MCP-1 水平（P<0.05 或 P<0.01）。EEAJ 的抗炎作用与 DEX 相似。
落葵可能是一种新的治疗药物，具有减少或替代皮质类固醇的潜力，其机制与 TNF-α 产生的调节密切相关。
