POLTAVA STATE MEDICAL UNIVERSITY, POLTAVA, UKRAINE.
POLTAVA V.G. KOROLENKO NATIONAL PEDAGOGICAL UNIVERSITY, POLTAVA, UKRAINE.
Wiad Lek. 2022;75(6):1466-1470. doi: 10.36740/WLek202206107.
The aim: Investigation of ultrastructural changes in the elements of rats' duodenal mucosa in norm and exposed to a complex of food additives (monosodium glutamate, sodium nitrite and Ponceau 4R).
Materials and methods: 70 rats of the experimental groups was administered 0.6 mg/kg of sodium nitrite, monosodium glutamate at a dose of 20 mg/kg, Ponceau 4R at a dose of 5 mg/kg in 0.5 ml of distilled water once daily per os. The doses of food additives were twice lower the allowable normal rate in food products. Animals were removed from the experiment at 1, 4, 8, 12 and 16 weeks.
Results: The effect of the complex of food additives on the mucous membrane of the duodenum was manifested by the development of edema and increased local immune response. In the later stages of observation, dystrophic changes in epithelial cells were determined. Vacuoles were found in the cytoplasm.
Conclusions: The use of a complex of food additives led to general ultramicroscopic changes in the mucous membrane of rats' duodenum, triggering the morphological mechanisms of nonspecific inflammation in the form of dystrophic changes and the development of apoptosis.
研究正常大鼠和暴露于复合食品添加剂（谷氨酸钠、亚硝酸钠和诱惑红 4R）的大鼠十二指肠黏膜各元素的超微结构变化。
材料和方法：实验组的 70 只大鼠每天经口给予 0.6mg/kg 亚硝酸钠、20mg/kg 谷氨酸钠和 5mg/kg 诱惑红 4R，溶于 0.5ml 蒸馏水中。食品添加剂的剂量是食品中允许正常剂量的两倍。动物在 1、4、8、12 和 16 周时从实验中取出。
结果：复合食品添加剂对十二指肠黏膜的影响表现为水肿和局部免疫反应增强。在观察的后期阶段，确定了上皮细胞的营养不良变化。细胞质中发现有空泡。
结论：复合食品添加剂的使用导致大鼠十二指肠黏膜的一般超微结构变化，引发非特异性炎症的形态学机制，表现为营养不良变化和细胞凋亡的发展。
