POLTAVA STATE MEDICAL UNIVERSITY, POLTAVA, UKRAINE.
Wiad Lek. 2022;75(6):1525-1528. doi: 10.36740/WLek202206117.
The aim: The aim of the paper was the experimental study of the morphological features of albino rat hepatocytes after the consumption of the complex of food additives (monosodium glutamate, sodium nitrite, Ponceau 4R) supplemented into the ration and consumed for four weeks.
Materials and methods: The study was performed on 30 outbred albino rats of both genders, weighing 204±0.67 g. The ration of the experimental animals, supplemented with a combination of food additives, namely, monosodium glutamate, Ponceau 4R, sodium nitrate, was consumed for 1 and 4 weeks. The study of the structure of hepathocytes was carried out on traditional histological preparations and preparations stained with Best's carmine.
Results: Supplementation of ration with the complex of food additives for one week showed the phenomena of fatty degeneration that dominated in hepatocytes, and in a longer consumption of food additives in the ration (for four weeks), the number of liver cells with the phenomena of hydropic degeneration significantly increased, while individual hepatocytes had signs of irreversible destructive changes.
Conclusions: Consumption of the complex of food additives supplemented into the standard ration of laboratory animals for 4 weeks leads to a significant change in the dimensions of the liver cells, a decrease in their glycogen content, and a progressive increase in the number of hepatocytes with alterations.
本文旨在研究食用添加物复合物（谷氨酸单钠、亚硝酸钠、诱惑红 4R）饲养四周后的白化大鼠肝细胞的形态学特征。
本研究使用了 30 只雌雄异体、体重为 204±0.67g 的白化大鼠。实验动物的饲料中补充了组合添加物，即谷氨酸单钠、诱惑红 4R、亚硝酸钠，分别喂养 1 周和 4 周。采用传统的组织学切片和 Best 胭脂红染色法对肝细胞的结构进行研究。
添加物复合物喂养 1 周表现出以肝细胞脂肪变性为主的现象，而在更长时间的添加物（4 周）喂养后，水样变性的肝细胞数量明显增加，而个别肝细胞出现不可逆性破坏改变。
4 周内食用添加物复合物饲养标准实验动物的饲料会导致肝细胞的尺寸发生显著变化，其糖原含量降低，伴有改变的肝细胞数量逐渐增加。
