Lu Xianlin, Zhan Yu, He Wei
School of Pharmacy, The Air Force Medical University, Xi'an 710032, PR China.
School of Pharmacy, The Air Force Medical University, Xi'an 710032, PR China.
J Photochem Photobiol B. 2022 Sep;234:112528. doi: 10.1016/j.jphotobiol.2022.112528. Epub 2022 Jul 18.
Fluorescence probes, as analytical tools with the ability to perform rapid and sensitive detection of target analytes, have made outstanding contributions to environmental analysis and bioassays. Considering the expanding developments in these areas, fluorophores play a key role in the de-sign of fluorescence probes. Compared to classical fluorophores, phenothiazines with elec-tron-rich characteristics have been widely applied to construct electron donor-acceptor dyes, which exhibit outstanding performance in both fluorimetric and colorimetric analysis. In addition, these probes also exhibit the pronounced ability in both solution and solid-state, achieving portable detection for environmental analysis. In this review, we summarize recent advances in the performance of phenothiazine-based fluorescent probes for detecting various analytes, especially in cations, anions, ROS/RSS, enzyme and other small molecules. The general design rules, response mechanisms and practical applications of the probes are analyzed, followed by a discussion of exiting challenges and future research perspectives. It is hoped that this review will provide a few strategies for the development of phenothiazine-based fluorescent probes.
荧光探针作为能够对目标分析物进行快速灵敏检测的分析工具，在环境分析和生物测定中做出了杰出贡献。鉴于这些领域的不断发展，荧光团在荧光探针的设计中起着关键作用。与传统荧光团相比，具有富电子特性的吩噻嗪已被广泛应用于构建电子供体-受体染料，这些染料在荧光分析和比色分析中均表现出优异性能。此外，这些探针在溶液和固态中也都表现出显著的能力，实现了环境分析的便携式检测。在本综述中，我们总结了基于吩噻嗪的荧光探针在检测各种分析物，特别是阳离子、阴离子、活性氧/活性硫、酶和其他小分子方面性能的最新进展。分析了探针的一般设计规则、响应机制和实际应用，随后讨论了现存挑战和未来研究前景。希望本综述能为基于吩噻嗪的荧光探针的开发提供一些策略。
