Ma Qiao, Liu Lei, Luo Chunfeng, Wu Yue, Qin Fengshu, Du Kai
Shenzhen New Industries Biomedical Engineering Co., Ltd, Reagent Key Raw Materials R&D and Production Center Pingshan District Shenzhen Guangdong 518122 P. R. China
RSC Adv. 2025 May 28;15(22):17776-17780. doi: 10.1039/d5ra00677e. eCollection 2025 May 21.
-(4-Aminobutyl)--ethylisoluminol (ABEI) as an efficient label is widely used in automated immunoassays, yet the labelling process of ABEI onto biomacromolecules like proteins is not straightforward as the transformation of an amino group into a carboxyl group is needed. We report the synthesis of isoluminol derivatives with a terminal carboxyl group for protein labelling. And also, an -hydroxysuccinimide active ester of carboxylic acid as a direct protein labelling reagent was isolated with high purity. This labelling reagent was proved to have good chemiluminescence efficiency, be stable and possess high labelling efficacy with BSA. The introduction of the propyl sulfonic group as a hydrophilic functional group increased not only water solubility but also the chemiluminescence quantum yields. In addition, non-specific binding of magnetic microparticles was also decreased notably when water soluble labelling reagents were used.
（4-氨基丁基）-N-乙基异鲁米诺（ABEI）作为一种高效标记物，广泛应用于自动化免疫分析中。然而，将ABEI标记到蛋白质等生物大分子上的过程并不简单，因为需要将氨基转化为羧基。我们报道了用于蛋白质标记的具有末端羧基的异鲁米诺衍生物的合成。此外，还分离出了高纯度的羧酸N-羟基琥珀酰亚胺活性酯作为直接蛋白质标记试剂。该标记试剂被证明具有良好的化学发光效率、稳定性，并且对牛血清白蛋白具有高标记效率。引入丙基磺酸基团作为亲水性官能团，不仅增加了水溶性，还提高了化学发光量子产率。此外，使用水溶性标记试剂时，磁性微粒的非特异性结合也显著降低。
