Key Laboratory of Functional Nanomaterials and Technology in Universities of Shandong, College of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, P. R. China.
J Mater Chem B. 2021 Jan 7;9(1):53-79. doi: 10.1039/d0tb01628d. Epub 2020 Nov 23.
The fluorescence imaging technique has attracted increasing attention in the detection of various biological molecules in situ and in real-time owing to its inherent advantages including high selectivity and sensitivity, outstanding spatiotemporal resolution and fast feedback. In the past few decades, a number of fluorescent probes have been developed for bioassays and imaging by exploiting different fluorophores. Among various fluorophores, resorufin exhibits a high fluorescence quantum yield, long excitation/emission wavelength and pronounced ability in both fluorescence and colorimetric analysis. This fluorophore has been widely utilized in the design of responsive probes specific for various bioactive species. In this review, we summarize the advances in the development of resorufin-based fluorescent probes for detecting various analytes, such as cations, anions, reactive (redox-active) sulfur species, small molecules and biological macromolecules. The chemical structures of probes, response mechanisms, detection limits and practical applications are investigated, which is followed by the discussion of recent challenges and future research perspectives. This review article is expected to promote the further development of resorufin-based responsive fluorescent probes and their biological applications.
荧光成像技术由于其固有优势，包括高选择性和灵敏度、出色的时空分辨率和快速反馈，在原位和实时检测各种生物分子方面引起了越来越多的关注。在过去的几十年中，已经开发了许多荧光探针用于生物分析和成像，利用了不同的荧光团。在各种荧光团中，resorufin 具有高荧光量子产率、长激发/发射波长以及在荧光和比色分析中都具有显著的能力。该荧光团已广泛用于设计针对各种生物活性物质的响应性探针。在这篇综述中，我们总结了基于 resorufin 的荧光探针在检测各种分析物（如阳离子、阴离子、反应性（氧化还原活性）硫物种、小分子和生物大分子）方面的发展。我们研究了探针的化学结构、响应机制、检测限和实际应用，随后讨论了最近的挑战和未来的研究展望。本文综述有望促进基于 resorufin 的响应性荧光探针及其生物应用的进一步发展。
