Biomedical Centre Martin JFM CU, Jessenius Faculty of Medicine in Martin (JFM CU), Comenius University in Bratislava, Slovakia.
Biomedical Centre Martin JFM CU, Jessenius Faculty of Medicine in Martin (JFM CU), Comenius University in Bratislava, Slovakia.
Stem Cell Res. 2022 Aug;63:102870. doi: 10.1016/j.scr.2022.102870. Epub 2022 Jul 13.
We present here a new iPS cell line for modeling sporadic form of ALS. Cell line was generated by reprogramming skin fibroblasts isolated with explant culture technology from skin biopsy, donated by ALS patient. For reprogramming, polycistronic self-replicating RNA vector was used and derived iPS cells were characterized by immunocytochemistry and FACS (pluripotent factors expression), karyotyping, STR fingerprinting analysis and in vitro differentiation assay. New cell line showed normal (46, XY) karyotype and differentiated in vitro into cells from three germ layers. STR analysis proved the origin and originality of the cell line.
我们在此介绍一种新的 iPS 细胞系，用于模拟散发性肌萎缩侧索硬化症。该细胞系是通过使用多顺反子自我复制 RNA 载体对从皮肤活检中分离出来的皮肤成纤维细胞进行重编程而产生的，这些成纤维细胞是由肌萎缩侧索硬化症患者捐赠的。通过免疫细胞化学和 FACS（多能性因子表达）、核型分析、STR 指纹分析和体外分化试验对衍生的 iPS 细胞进行了特征鉴定。新的细胞系显示出正常的（46, XY）核型，并在体外分化为三个胚层的细胞。STR 分析证明了该细胞系的起源和独特性。
