Aiming at enhancing the biosensing detection of histamine in foods at high temperature, we developed a thermoregulatory biosensor based on diamine oxidase-immobilized phase-change microcapsules consisting of an n-docosane core, a TiO shell, and an electroactive polyaniline/ZnO composite coating layer. The microcapsules exhibit a satisfactory latent heat capacity of over 112 J/g for thermo-temperature regulation. Through an innovative integration of electroactive phase-change microcapsules and biological enzyme in the working electrode, the biosensor obtained a thermoregulatory function through reversible phase transitions by the n-docosane core under high-temperature environments. This enables the biosensor to achieve a higher response sensitivity of 28.57 µA⋅mM⋅cm and a lower detection limit of 0.473 µmol/L at the high assay temperatures compared to conventional histamine biosensors. With enhanced electrochemical biosensing performance through in-situ thermo-temperature regulation, the smart biosensor developed in this study has found practical applications for high-sensitive detection and high-accurate quantitive determination of histamine in foods across a broad temperature range.