Department of Veterinary Medicine & Institute of Veterinary Science, Chungnam National University, Daejeon 34134, Republic of Korea.
Department of Veterinary Medicine & Institute of Veterinary Science, Chungnam National University, Daejeon 34134, Republic of Korea.
Chin J Nat Med. 2022 Jul;20(7):518-526. doi: 10.1016/S1875-5364(22)60178-X.
Benign prostatic hyperplasia (BPH) is a chronic male disease characterized by the enlarged prostate. Celtis chosenianaNakai (C. choseniana) is medicinally used to alleviate pain, gastric disease, and lung abscess. In this study, the effect of C. choseniana extract on BPH was investigated using testosterone-induced rats. Sprague Dawley rats were divided into five groups: control, BPH (testosterone 5 mg·kg), Fina (finasteride 2 mg·kg), and C. choseniana (50 and 100 mg·kg). After four weeks of TP treatment with finasteride or C. choseniana, prostate weights and DHT levels were measured. In addition, the prostates were histopathologically examined and measured for protein kinase B (Akt)/nuclear factor-κB (NF-κB)/AR signaling, proliferation, apoptosis, and autophagy. Prostate weight and epithelial thickness were reduced in the C. choseniana groups compared with that in the BPH group. The extract of C. choseniana acted as a 5α reductase inhibitor, reducing DHT levels in the prostate. Furthermore, the extract of C. choseniana blocked the activation of p-Akt, nuclear NF-κB activation and reduced the expression of AR and PSA compared with BPH. Moreover, the expression of Bax, PARP-1, and p53 increased, while the expression of bcl-2 decreased. The present study demonstrated that C. choseniana extract alleviated testosterone-induced BPH by suppressing 5α reductase and Akt/NF-κB activation, reducing AR signaling and inducing apoptosis and autophagy in the prostate. These results suggested that C. choseniana probably contain potential herbal agents to alleviate BPH.
良性前列腺增生症（BPH）是一种慢性男性疾病，其特征为前列腺增大。川朴（ CeltischosenianaNakai）被用作药物来缓解疼痛、胃病和肺脓肿。在这项研究中，使用睾丸素诱导的大鼠来研究川朴提取物对 BPH 的影响。SD 大鼠分为五组：对照组、BPH（睾丸素 5mg·kg）、非那雄胺（finasteride，2mg·kg）和川朴（50 和 100mg·kg）。用 finasteride 或川朴处理大鼠 4 周后，测量前列腺重量和 DHT 水平。此外，还对前列腺进行了组织病理学检查，并测量了蛋白激酶 B（Akt）/核因子-κB（NF-κB）/雄激素受体（AR）信号、增殖、凋亡和自噬。与 BPH 组相比，川朴组的前列腺重量和上皮厚度降低。川朴提取物作为 5α 还原酶抑制剂，降低了前列腺中的 DHT 水平。此外，川朴提取物阻断了 p-Akt 的激活、核 NF-κB 的激活，并降低了 AR 和 PSA 的表达，与 BPH 相比。此外，Bax、PARP-1 和 p53 的表达增加，而 bcl-2 的表达减少。本研究表明，川朴提取物通过抑制 5α 还原酶和 Akt/NF-κB 的激活，降低 AR 信号并诱导前列腺细胞凋亡和自噬，从而缓解睾丸素诱导的 BPH。这些结果表明，川朴可能含有潜在的草药制剂来缓解 BPH。
