Division of Food Bioscience, College of Biomedical and Health Sciences, Konkuk University, Chungju 27478, Korea.
School of Bio-science and Food Engineering, Changchun University of Science and Technology, Changchun 130-600, China.
Int J Environ Res Public Health. 2019 Oct 7;16(19):3764. doi: 10.3390/ijerph16193764.
Benign prostatic hyperplasia (BPH) is one of the major public health concerns, which has a high prevalence rate and causes significant decline in men's quality of life. BPH is highly related to sexual hormone metabolism and aging. In particular, dihydrotestosterone (DHT), to which testosterone is modified by 5α-reductase (5AR), has a significant effect on BPH development. DHT binds to an androgen receptor (AR) and steroid receptor coactivator 1 (SRC-1); then, it induces the proliferation of a prostate cell and expression of prostate specific antigen (PSA). () is a mushroom that has been popularized by the artificial cultivation of fruiting bodies based on silkworms by researchers from the Republic of Korea. In a previous study, we identified the effect of PE on PSA mRNA expression in LNCaP cells. This suggests that PE may have an inhibitory effect on androgen signaling. Therefore, we confirmed the expression of androgen signaling-related factors, such as AR, SRC-1, and PSA in LNCaP. Furthermore, we confirmed the androgen signaling inhibitory effect of PE using the testosterone propionate (TP)-induced BPH rat model. A BPH rat model was established with a four-week treatment of daily subcutaneous injections of testosterone propionate (TP, 3 mg/kg) dissolved in corn oil after castration. The rats in the treatment group were orally gavaged extract (PE), finasteride (Fi), or saw palmetto extract (Saw) with TP injection. DHT induced an increase in the expression levels of AR, SRC-1, and PSA proteins in LNCaP cells. On the contrary, the PE treatment reduced the expression levels. In vivo the BPH group showed an increase in prostate size compared with the control group. The PE gavaged group showed a decrease in prostate size compared with the BPH group. In addition, the protein expressions of AR, 5AR2, and PSA were significantly lower in the PE gavaged group than BPH group in prostate tissue. These results suggest the beneficial effects of PE on BPH via the modulation of AR signaling pathway.
良性前列腺增生症 (BPH) 是一个主要的公共健康问题，它具有较高的患病率，并导致男性生活质量显著下降。BPH 与性激素代谢和衰老密切相关。特别是二氢睾酮 (DHT)，它是由 5α-还原酶 (5AR) 对睾酮进行修饰而成的，对 BPH 的发展有显著影响。DHT 与雄激素受体 (AR) 和类固醇受体共激活剂 1 (SRC-1) 结合；然后，它诱导前列腺细胞的增殖和前列腺特异性抗原 (PSA) 的表达。 (PE) 是一种蘑菇，韩国研究人员通过人工培育蚕蛹的方法使其在果实中大量繁殖。在之前的研究中，我们发现了 PE 对 LNCaP 细胞中 PSA mRNA 表达的影响。这表明 PE 可能对雄激素信号有抑制作用。因此，我们在 LNCaP 细胞中证实了雄激素信号相关因子，如 AR、SRC-1 和 PSA 的表达。此外，我们使用丙酸睾酮 (TP) 诱导的 BPH 大鼠模型证实了 PE 的雄激素信号抑制作用。BPH 大鼠模型是通过在去势后每天用溶解在玉米油中的丙酸睾酮 (TP，3mg/kg) 进行皮下注射四周来建立的。治疗组大鼠口服给予 PE、非那雄胺 (Fi) 或锯棕榈提取物 (Saw) 与 TP 注射。DHT 诱导 LNCaP 细胞中 AR、SRC-1 和 PSA 蛋白表达水平增加。相反，PE 处理降低了表达水平。在体内，BPH 组与对照组相比，前列腺体积增大。与 BPH 组相比，PE 灌胃组前列腺体积减小。此外，在前列腺组织中，PE 灌胃组的 AR、5AR2 和 PSA 蛋白表达明显低于 BPH 组。这些结果表明，PE 通过调节 AR 信号通路对 BPH 有有益的影响。
